A Photo-Triggered and Photo-Calibrated Nitric Oxide Donor: Rational Design, Spectral Characterizations, and Biological Applications

He, Haihong, Liu, Yuxin, Zhou, Zhongneng, Guo, Chunlei, Wang, Hong-Yin, Wang, Zhuang, Wang, Xueli, Zhang, Ziqian, Wu, Fu-Gen, Wang, Haolu, Chen, Daijie, Yang, Dahai, Liang, Xiaowen, Chen, Jinquan, Zhou, Shengmin, Liang, Xin, Qian, Xuhong and Yang, Youjun (2018) A Photo-Triggered and Photo-Calibrated Nitric Oxide Donor: Rational Design, Spectral Characterizations, and Biological Applications. Free radical biology & medicine, . doi:10.1016/j.freeradbiomed.2018.04.563


Author He, Haihong
Liu, Yuxin
Zhou, Zhongneng
Guo, Chunlei
Wang, Hong-Yin
Wang, Zhuang
Wang, Xueli
Zhang, Ziqian
Wu, Fu-Gen
Wang, Haolu
Chen, Daijie
Yang, Dahai
Liang, Xiaowen
Chen, Jinquan
Zhou, Shengmin
Liang, Xin
Qian, Xuhong
Yang, Youjun
Title A Photo-Triggered and Photo-Calibrated Nitric Oxide Donor: Rational Design, Spectral Characterizations, and Biological Applications
Journal name Free radical biology & medicine   Check publisher's open access policy
ISSN 1873-4596
Publication date 2018-04-27
Sub-type Article (original research)
DOI 10.1016/j.freeradbiomed.2018.04.563
Open Access Status Not yet assessed
Abstract Nitric oxide (NO) donors are valuable tools to probe the profound implications of NO in health and disease. The elusive nature of NO bio-relevance has largely limited the use of spontaneous NO donors and promoted the development of next generation NO donors, whose NO release is not only stimulated by a trigger, but also readily monitored via a judiciously built-in self-calibration mechanism. Light is without a doubt the most sensitive, versatile and biocompatible method of choice for both triggering and monitoring, for applications in complex biological matrices. Herein, we designed and synthesized an N-nitroso rhodamine derivative (NOD560) as a photo-triggered and photo-calibrated NO donor to address this need. NOD560 is essentially non-fluorescent. Upon irradiation by green light (532nm), it efficiently release NO and a rhodamine dye, the dramatic fluorescence turn-on from which could be harnessed to conveniently monitor the localization, flux, and dose of NO release. The potentials of NOD560 for in vitro biological applications were also exemplified in in vitro biological models, i.e. mesenchymal stem cell (MSC) migration suppression. NOD560 is expected to complement the existing NO donors and find widespread applications in chemical biological studies.
Keyword Nitrc oxide donors
mesenchymal stem cell migration
photo-calibrated
photo-triggered
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Pubmed Import
 
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Created: Wed, 09 May 2018, 10:11:13 EST