The Effect Of Draw-Up Volume On The Accuracy Of Electrolyte Measurements From Neonatal Arterial Lines

Davies, M. W., Mehr, S. and Morley, C. J. (2000) The Effect Of Draw-Up Volume On The Accuracy Of Electrolyte Measurements From Neonatal Arterial Lines. Journal of Paediatrics and Child Health, 36 2: 122-124. doi:10.1046/j.1440-1754.2000.00451.x

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Author Davies, M. W.
Mehr, S.
Morley, C. J.
Title The Effect Of Draw-Up Volume On The Accuracy Of Electrolyte Measurements From Neonatal Arterial Lines
Journal name Journal of Paediatrics and Child Health   Check publisher's open access policy
ISSN 1034-4810
1440-1754
Publication date 2000-04-01
Year available 2000
Sub-type Article (original research)
DOI 10.1046/j.1440-1754.2000.00451.x
Open Access Status File (Author Post-print)
Volume 36
Issue 2
Start page 122
End page 124
Total pages 3
Editor Oberklaid, Frank
Place of publication Melbourne
Publisher Blackwell Scientific
Language eng
Subject 321019 Paediatrics
Abstract Objectives: Contamination by infusate of blood samples withdrawn from arterial lines has been recognized but not well documented for neonates. The aim of this study was to investigate, using in vitro and in vivo studies, the effects of different draw-up volumes (withdrawn from the line prior to the sample being taken) on the concentration of sodium. Methods: In-vitro study: The tip of an umbilical artery catheter (dead space 0.6 mL), infused with half normal saline containing 1 unit/mL of heparin was placed in a beaker of normal saline. The line was flushed with 1 mL of this infusate just before each sample was taken. Volumes from 0.5 mL to 2.0 mL of infusate/normal saline were withdrawn in 0.1 mL increments from a three-way tap and discarded. A sample was then taken from the line into a blood gas syringe for analysis of the sodium concentration by the 860 Blood Gas Analyzer (Chiron Diagnostics, Bayer, Scoresby). Control samples were taken from the beaker. In-vivo study: A 22 gauge intravenous catheter was inserted into a vein of an adult male volunteer. The dead space was also 0.6 mL. The line was flushed with 5 mL of half-normal saline immediately before sampling. Draw-up volumes of 0.6, 0.9, 1.3, and 1.6 mL were withdrawn and discarded. 10 mL was used as a control. A 0.5-mL blood sample was then taken and the electrolyte concentrations analysed immediately. Results: In-vitro: A minimum draw-up volume of 1.3 mL was required before the sodium concentration was not significantly different from the control samples. In-vivo: A minimum draw-up volume of 1.6 mL was required before the sodium concentration was not significantly different from the control samples. There were similar trends in the effect of draw-up volume for glucose, calcium, potassium, chloride and lactate. Conclusion: A minimum volume of 1.6 mL should be withdrawn from neonatal arterial lines (dead space 0.6 mL) before taking blood for analysis
Keyword arterial cannula
blood specimen collection
infant
newborn
sodium concentration
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown
Additional Notes Originally published as MW Davies, S. Mehr and CJ Morley (2000) The effect of draw-up volume on the accuracy of electrolyte measurements from neonatal arterial lines, J. Paediatr. Child Health, 36 (2): 122-124. Copyright 2000 Blackwell Publishing. All rights reserved.

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Medicine Publications
 
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Created: Fri, 07 Jan 2005, 10:00:00 EST