Progress in the development of transgenic papaya: transformation of solo papaya using ACC synthase antisense construct

Magdalita, P. M., Laurena, A. C., Yabut-Perez, B.M., Tecson-Mendoza, E. M., Villegas, V. N. and Botella, J. R. (2002). Progress in the development of transgenic papaya: transformation of solo papaya using ACC synthase antisense construct. In: R. Drew et al., Proceedings of the International Symposium on Tropical and Subtropical Fruits. The International Symposium on Tropical and Subtropical Fruits, Cairns, Australia, (171-176). 26 Nov - 1 Dec 2000. doi:10.17660/ActaHortic.2002.575.17


Author Magdalita, P. M.
Laurena, A. C.
Yabut-Perez, B.M.
Tecson-Mendoza, E. M.
Villegas, V. N.
Botella, J. R.
Title of paper Progress in the development of transgenic papaya: transformation of solo papaya using ACC synthase antisense construct
Conference name The International Symposium on Tropical and Subtropical Fruits
Conference location Cairns, Australia
Conference dates 26 Nov - 1 Dec 2000
Proceedings title Proceedings of the International Symposium on Tropical and Subtropical Fruits   Check publisher's open access policy
Journal name Proceedings of the International Symposium On Tropical and Subtropical Fruits, Vols 1 and 2   Check publisher's open access policy
Place of Publication Leuven, Belgium
Publisher ISHS
Publication Year 2002
Year available 2002
Sub-type Fully published paper
DOI 10.17660/ActaHortic.2002.575.17
Open Access Status Not yet assessed
ISBN 90-6605-885-4
ISSN 0567-7572
Editor R. Drew et al.
Volume 1
Issue 575
Start page 171
End page 176
Total pages 6
Language eng
Abstract/Summary Papaya (cv. Solo) somatic embryos were produced, squashed and used for transient stable transformation. Conditions for transforming somatic embryos were optimized using transient expression of GUS. A total of 11,900 immature zygotic embryos were isolated and 7,730 (65%) produced somatic embryos. All the somatic embryos were squashed, placed in an osmotic medium and bombarded with the antisense ACC synthase construct placed in a co-transformation vector. A total of 160 bombardments were made. The bombarded tissues were subjected to a selection medium containing half-strength MS with 150 mg L-1, then with 300 mg L-1 kanamycin. Embryos surviving kanamycin-selection were regenerated on a full strength De Fossard medium supplemented with 0.25 muM each of BAP and NAA and 10.0 muM GA(3).
Subjects E1
270800 Biotechnology
620200 Horticultural Crops
Keyword Agronomy
Biotechnology & Applied Microbiology
Horticulture
Agriculture
Biotechnology & Applied Microbiology
Q-Index Code E1
Institutional Status Non-UQ

Document type: Conference Paper
Sub-type: Fully published paper
Collections: Excellence in Research Australia (ERA) - Collection
School of Biological Sciences Publications
 
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Created: Fri, 24 Aug 2007, 10:55:50 EST