Clonging and molecular characterization of ripening-related ACC synthase from papaya fruit (Carica papaya L.)

Laurena, A. C., Magdalita, P. M., Hidalgo, M. S. P., Villegas, V. N., Mendoza, E. M. T. and Botella, J. R. (2002). Clonging and molecular characterization of ripening-related ACC synthase from papaya fruit (Carica papaya L.). In: R. Drew et al., Proceedings of the International Symposium on Tropical and Subtropical Fruits. The International Symposium on Tropical and Subtropical Fruits, Cairns, Australia, (163-169). 26 Nov - 1 Dec 2000. doi:10.17660/ActaHortic.2002.575.16


Author Laurena, A. C.
Magdalita, P. M.
Hidalgo, M. S. P.
Villegas, V. N.
Mendoza, E. M. T.
Botella, J. R.
Title of paper Clonging and molecular characterization of ripening-related ACC synthase from papaya fruit (Carica papaya L.)
Conference name The International Symposium on Tropical and Subtropical Fruits
Conference location Cairns, Australia
Conference dates 26 Nov - 1 Dec 2000
Proceedings title Proceedings of the International Symposium on Tropical and Subtropical Fruits   Check publisher's open access policy
Journal name Proceedings of the International Symposium On Tropical and Subtropical Fruits, Vols 1 and 2   Check publisher's open access policy
Place of Publication Leuven, Belgium
Publisher ISHS
Publication Year 2002
Year available 2002
Sub-type Fully published paper
DOI 10.17660/ActaHortic.2002.575.16
Open Access Status Not yet assessed
ISBN 90-6605-885-4
ISSN 0567-7572
Editor R. Drew et al.
Volume 1
Issue 575
Start page 163
End page 169
Total pages 7
Language eng
Abstract/Summary Papaya is a climacteric fruit and like other climacteric fruits in the tropics, one of the main problems is the significant post-harvest loss due to uncontrolled ripening. One molecular strategy to control ripening after the climacteric rise in ethylene is to down-regulate ethylene biosynthesis through antisense expression of the ripening-related ACC synthase gene. Total RNA was isolated from 100% ripe papaya fruit and reverse transcriptase polymerase chain reaction (RT-PCR) was used to amplify ripening-related ACC synthase cDNAs. Two pairs of degenerate primers (oligonucleotides) derived from conserved regions of ACC synthase from several plant sources were used for RT-PCR. Two different members of the ACC synthase multigene family were isolated and characterized from ripe tissues of papaya whose expression patterns differ greatly from each other. The two ACC synthase cDNAs, ACS1 and ACS2 differ from each other in terms of restriction digest patterns, DNA and amino acid sequence analysis and expression during ripening. ACS2 expression pattern in papaya cv Solo Kapoho exhibits a striking similarity to that of leacs2 in tomato whose transcript levels are high during ripening. ACS2 is a good candidate gene of ACC synthase for antisense strategies to regulate or control fruit ripening. ACS2 was inserted in an antisense orientation in two types of vector constructs for papaya transformation via particle bombardment.
Subjects E1
270800 Biotechnology
620200 Horticultural Crops
Keyword Agronomy
Biotechnology & Applied Microbiology
Horticulture
Agriculture
Biotechnology & Applied Microbiology
Q-Index Code E1
Institutional Status Non-UQ

Document type: Conference Paper
Sub-type: Fully published paper
Collections: Excellence in Research Australia (ERA) - Collection
School of Biological Sciences Publications
 
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Created: Fri, 24 Aug 2007, 10:55:44 EST