Cellular and Cytokine Correlates of Mucosal Protection in Murine Model of Oral Candidiasis

Elahi, Shokrollah, Pang, Gerald, Clancy, Robert and Ashman, Robert B. (2000) Cellular and Cytokine Correlates of Mucosal Protection in Murine Model of Oral Candidiasis. Infection and Immunity, 68 10: 5771-5777. doi:10.1128/IAI.68.10.5771-5777.2000

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Author Elahi, Shokrollah
Pang, Gerald
Clancy, Robert
Ashman, Robert B.
Title Cellular and Cytokine Correlates of Mucosal Protection in Murine Model of Oral Candidiasis
Journal name Infection and Immunity
ISSN 1098-5522
1070-6313
Publication date 2000-10-01
Sub-type Article (original research)
DOI 10.1128/IAI.68.10.5771-5777.2000
Open Access Status File (Publisher version)
Volume 68
Issue 10
Start page 5771
End page 5777
Total pages 7
Place of publication Washington, DC, United States
Publisher American Society of Microbiology
Collection year 2000
Language eng
Abstract Host protection against Candida albicans infection in a model of oral candidiasis involving infection-prone [DBA/2 (H-2d)] and less infection-prone [BALB/c (H-2d)] mouse strains was analyzed in terms of antibody and cellular responses, and in terms of cytokine patterns from regional lymph node cells. There was a selective expansion of g/d1 T-cell receptor cells, which correlated with the patterns of colonization in both mouse strains, with higher numbers of g/d T cells detected in BALB/c mice. Antigen-induced T-cell proliferation was significantly higher in BALB/c mice than in DBA/2 mice. Higher levels of serum immunoglobulin G (IgG) and salivary IgA antibodies were detected in BALB/c mice than in DBA/2 mice, but only after the infection was cleared. The cervical lymph node cells from infected mice were assessed for interleukin-4 (IL-4), IL-12, and gamma interferon (IFN-g) mRNA gene expression by reverse transcription-PCR and protein production in the culture supernatants following restimulation in vitro. In BALB/c mice, an early increase in levels of IL-4, IFN-g, and IL-12 correlated with rapid elimination of C. albicans. In DBA/2 mice, where resolution of infection was delayed, IL-4 message expression was delayed and the IL-4 secretion level was lower. Neutralization of IL-4 by multiple injections of an anti-IL-4 monoclonal antibody in BALB/c mice resulted in increased carriage rate and delayed clearance of the yeasts. Collectively, the data suggest that the T-cell response to C. albicans in the regional lymph nodes which correlates best with rapid oral clearance of C. albicans is a balanced Th0 cytokine response involving early secretion of both IFN-g and IL-4.0
Keyword Candida
mucosa
cytokines
T cells
Q-Index Code C1
Institutional Status UQ
Additional Notes Originally published as Elahi, S. and Pang, G. and Clancy, R. and Ashman, R.B. (2000) Cellular and cytokine correlates of mucosal protection in murine model of oral candidiasis. Infection and Immunity 68(10):5771-5777. Copyright 2000 The American Society for Microbiology. All rights reserved.

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
School of Dentistry Publications
 
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