Calcium channels controlling acetylcholine release from preganglionic nerve terminals in rat autonomic ganglia

Smith, A. B., Motin, L., Lavidis, N. A. and Adams, D. J. (1999) Calcium channels controlling acetylcholine release from preganglionic nerve terminals in rat autonomic ganglia. Neuroscience, 95 4: 1121-1127. doi:10.1016/S0306-4522(99)00505-9

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Author Smith, A. B.
Motin, L.
Lavidis, N. A.
Adams, D. J.
Title Calcium channels controlling acetylcholine release from preganglionic nerve terminals in rat autonomic ganglia
Journal name Neuroscience   Check publisher's open access policy
ISSN 0306-4522
Publication date 1999-12-01
Sub-type Article (original research)
DOI 10.1016/S0306-4522(99)00505-9
Open Access Status File (Author Post-print)
Volume 95
Issue 4
Start page 1121
End page 1127
Total pages 7
Editor Ottersen , O. P.
Place of publication Oxford, UK
Publisher Pergamon-Elsevier Science Ltd
Language eng
Subject 320700 Neurosciences
C1
320701 Autonomic Nervous System
730104 Nervous system and disorders
780105 Biological sciences
Abstract Little is known about the nature of the calcium channels controlling neurotransmitter release from preganglionic parasympathetic nerve fibres. In the present study, the effects of selective calcium channel antagonists and amiloride were investigated on ganglionic neurotransmission. Conventional intracellular recording and focal extracellular recording techniques were used in rat submandibular and pelvic ganglia, respectively. Excitatory postsynaptic potentials and excitatory postsynaptic currents preceded by nerve terminal impulses were recorded as a measure of acetylcholine release from parasympathetic and sympathetic preganglionic fibres following nerve stimulation. The calcium channel antagonists omega-conotoxin GVIA (N type), nifedipine and nimodipine (L type), omega-conotoxin MVIIC and omega-agatoxin IVA (P/Q type), and Ni2+ (R type) had no functional inhibitory effects on synaptic transmission in both submandibular and pelvic ganglia. The potassium-sparing diuretic, amiloride, and its analogue, dimethyl amiloride, produced a reversible and concentration-dependent inhibition of excitatory postsynaptic potential amplitude in the rat submandibular ganglion. The amplitude and frequency of spontaneous excitatory postsynaptic potentials and the sensitivity of the postsynaptic membrane to acetylcholine were unaffected by amiloride. In the rat pelvic ganglion, amiloride produced a concentration-dependent inhibition of excitatory postsynaptic currents without causing any detectable effects on the amplitude or configuration of the nerve terminal impulse. These results indicate that neurotransmitter release from preganglionic parasympathetic and sympathetic nerve terminals is resistant to inhibition by specific calcium channel antagonists of N-, L-, P/Q- and R-type calcium channels. Amiloride acts presynaptically to inhibit evoked transmitter release, but does not prevent action potential propagation in the nerve terminals, suggesting that amiloride may block the pharmacologically distinct calcium channel type(s) on rat preganglionic nerve terminals. (C) 1999 IBRO. Published by Elsevier Science Ltd.
Keyword Neurosciences
Neurotransmitter Release
Calcium Channels
Preganglionic Nerves
Acetylcholine
Amiloride
Calcium Channel Blockers
Omega-conotoxin Gvia
Transmitter Release
Vas-deferens
Pharmacological Properties
Neurotransmitter Release
Agatoxin-iva
P-type
T-type
Neurons
Inhibition
Q-Index Code C1
Institutional Status UQ
Additional Notes This is an author version of an article originally published as Smith, A. B., Motin, L., Lavidis, N. A. and Adams, D. J. (1999). Calcium channels controlling acetylcholine release from preganglionic nerve terminals in rat autonomic ganglia. Neuroscience, 95 (4), 1121-1127.doi:10.1016/S0306-4522(99)00505-9 Copyright 1999 Pergamon Press. All rights reserved. Single copies only may be downloaded and printed for a user’s personal research and study.

Document type: Journal Article
Sub-type: Article (original research)
Collection: Queensland Brain Institute Publications
 
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Created: Mon, 20 Aug 2007, 01:20:13 EST