Determination of organ tropism of Newcastle disease virus (strain I-2) by virus isolation and reverse transcription-polymerase chain reaction

Wambura, P, Meers, J and Spradbrow, P (2006) Determination of organ tropism of Newcastle disease virus (strain I-2) by virus isolation and reverse transcription-polymerase chain reaction. Veterinary Research Communications, 30 6: 697-706. doi:10.1007/s11259-006-3291-7


Author Wambura, P
Meers, J
Spradbrow, P
Title Determination of organ tropism of Newcastle disease virus (strain I-2) by virus isolation and reverse transcription-polymerase chain reaction
Journal name Veterinary Research Communications   Check publisher's open access policy
ISSN 0165-7380
Publication date 2006-01-01
Sub-type Article (original research)
DOI 10.1007/s11259-006-3291-7
Volume 30
Issue 6
Start page 697
End page 706
Total pages 10
Editor A van Miert
Place of publication Netherlands
Publisher springer-Verlag
Language eng
Subject C1
300510 Virology
780105 Biological sciences
Abstract The vaccines 1-2 and V4 are avirulent strains of Newcastle disease virus. Organ tropism of strain V4 has been determined and the virus has a predilection for the digestive tract. Tropism of strain 1-2 has not yet been determined. The objective of this study was to determine the distribution of strain 1-2 in various body organs and fluids following vaccination in comparison with V4. Four-week-old chickens were vaccinated by eye drop separately with these two avirulent strains. Virus isolation and the reverse transcription-polymerase chain reaction technique were employed to detect 1-2 and V4 viruses in various tissues and body fluids for 7 days following vaccination. Tissues from the respiratory tract showed earlier positive signals than tissues from other organs for chickens vaccinated with strain 1-2. Conversely, tissues from mainly digestive tract produced earlier positive signals than from respiratory tract and other organs from chickens vaccinated with strain V4. In early infection, strain 1-2 had preferential predilection for the respiratory tract and strain V4 for the digestive tract. Later after vaccination, other organs showed positive results from chickens vaccinated with both 1-2 and V4 strains. The differences in organ tropism observed in this study suggest that 1-2 may perform better than V4 as a live vaccine strain.
Keyword Newcastle Disease Virus
Reverse
Transcription-polymerase
Chain
Reaction
Veterinary Sciences
Chickens
Newcastle Disease
Reverse Transcription-polymerase Chain Reaction
Strain I-2
Strain V4
Tropism
Vaccine
Cleavage
Pathogenicity
Glycoproteins
Virulence
Protein
Site
Pcr
Q-Index Code C1

 
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Created: Wed, 15 Aug 2007, 20:17:27 EST