Using urea dilution to standardise cellular and non-cellular components of pleural and bronchoalveolar lavage (BAL) fluids in the cat

Mills, PC and Litster, A (2006) Using urea dilution to standardise cellular and non-cellular components of pleural and bronchoalveolar lavage (BAL) fluids in the cat. Journal of Feline Medicine And Surgery, 8 2: 105-110. doi:10.1016/j.jfms.2005.11.001


Author Mills, PC
Litster, A
Title Using urea dilution to standardise cellular and non-cellular components of pleural and bronchoalveolar lavage (BAL) fluids in the cat
Journal name Journal of Feline Medicine And Surgery   Check publisher's open access policy
ISSN 1098-612X
Publication date 2006-01-01
Sub-type Article (original research)
DOI 10.1016/j.jfms.2005.11.001
Volume 8
Issue 2
Start page 105
End page 110
Total pages 6
Editor A.H. Sparkes; M. Scherk
Place of publication UK
Publisher W.B. Saunders Co. Ltd.
Language eng
Subject C1
300512 Pharmacology
780105 Biological sciences
Abstract A technique to standardise the analysis of cellular and non-cellular components in epithelial lining fluid (ELF) collected during saline lavage of pulmonary and pleural cavities was developed using the urea dilution method. Bronchoalveolar lavage (BAL) and pleural lavage (PL) fluids were collected from 12 clinically healthy cats. Total and differential cell counts in BAL fluid were within normal ranges for the cat, while cell Counts in PL fluid were assumed to be normal based on clinical health during examination, auscultation and lactate dehydrogenase (LDH) activities being comparable with other species. The major clinical implication of this study was that nucleated cell counts within feline ELF could not be predicted from analysis of lavage fluid which suggests that calculation of the proportion of ELF in lavage fluid by the urea dilution method may be necessary to avoid misdiagnosis of health or disease in pulmonary or pleural cavities. (C) 2005 ESFM and AAFP. Published by Elsevier Ltd. All rights reserved.
Keyword Cellular
Non-cellular Components
Pleural
Bronchoalveolar Lavage
Cats
Veterinary Sciences
Epithelial Lining Fluid
Of-the-art
Lactate-dehydrogenase
Pulmonary-disease
Effector-cells
Dog
Effusions
Eosinophils
Disorders
Healthy
Q-Index Code C1

 
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Created: Wed, 15 Aug 2007, 20:05:55 EST