Silencing of the ACC synthase gene ACACS2 causes delayed flowering in pineapple [Ananas comosus (L.) Merr.]

Trusov, Y. and Botella, J. R. (2006) Silencing of the ACC synthase gene ACACS2 causes delayed flowering in pineapple [Ananas comosus (L.) Merr.]. Journal of Experimental Botany, 57 14: 3953-3960. doi:10.1093/jxb/erl167


Author Trusov, Y.
Botella, J. R.
Title Silencing of the ACC synthase gene ACACS2 causes delayed flowering in pineapple [Ananas comosus (L.) Merr.]
Journal name Journal of Experimental Botany   Check publisher's open access policy
ISSN 0022-0957
Publication date 2006-01-01
Year available 2006
Sub-type Article (original research)
DOI 10.1093/jxb/erl167
Open Access Status Not yet assessed
Volume 57
Issue 14
Start page 3953
End page 3960
Total pages 8
Place of publication Oxford
Publisher Oxford University Press
Language eng
Subject C1
270403 Plant Pathology
620205 Tropical fruit
Abstract Flowering is a crucial developmental stage in the plant life cycle. A number of different factors, from environmental to chemical, can trigger flowering. In pineapple, and other bromeliads, it has been proposed that flowering is triggered by a small burst of ethylene production in the meristem in response to environmental cues. A 1-amino-cyclopropane-1-carboxylate synthase (ACC synthase) gene has been cloned from pineapple (ACACS2), which is induced in the meristem under the same environmental conditions that induce flowering. Two transgenic pineapple lines have been produced containing co-suppression constructs designed to down-regulate the expression of the ACACS2 gene. Northern analysis revealed that the ACACS2 gene was silenced in a number of transgenic plants in both lines. Southern hybridization revealed clear differences in the methylation status of silenced versus non-silenced plants by the inability of a methylation-sensitive enzyme to digest within the ACACS2 DNA extracted from silenced plants, indicating that methylation is the cause of the observed co-suppression of the ACACS2 gene. Flowering characteristics of the transgenic plants were studied under field conditions in South East Queensland, Australia. Flowering dynamics studies revealed significant differences in flowering behaviour, with transgenic plants exhibiting silencing showing a marked delay in flowering when compared with non-silenced transgenic plants and control non-transformed plants. It is argued that the ACACS2 gene is one of the key contributors towards triggering 'natural flowering' in mature pineapples under commercial field conditions.
Keyword Acc Synthase
Co-suppression
Ethylene
Pineapple Flowering
Transgenic Pineapple
Plant Sciences
Smooth Cayenne Pineapple
1-aminocyclopropane-1-carboxylic Acid
Inflorescence Development
Ethylene Biosynthesis
Somaclonal Variation
Multigene Family
Growth
Plant
Dna
Induction
Q-Index Code C1
Institutional Status UQ

 
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Created: Wed, 15 Aug 2007, 19:47:17 EST