Transition metal complexes as mediator-titrants in protein redox potentiometry

Bernhardt, P. V., Chen, K. I. and Sharpe, P. C. (2006) Transition metal complexes as mediator-titrants in protein redox potentiometry. Journal of Biological Inorganic Chemistry, 11 7: 930-936. doi:10.1007/s00775-006-0148-z


Author Bernhardt, P. V.
Chen, K. I.
Sharpe, P. C.
Title Transition metal complexes as mediator-titrants in protein redox potentiometry
Journal name Journal of Biological Inorganic Chemistry   Check publisher's open access policy
ISSN 0949-8257
Publication date 2006-01-01
Sub-type Article (original research)
DOI 10.1007/s00775-006-0148-z
Volume 11
Issue 7
Start page 930
End page 936
Total pages 7
Place of publication New York
Publisher Springer
Language eng
Subject C1
250201 Transition Metal Chemistry
780103 Chemical sciences
Abstract A selection of nine macrocyclic Fe-III/II and Co-III/II transition metal complexes has been chosen to serve as a universal set of mediator-titrants in redox potentiometry of protein samples. The potential range spanned by these mediators is approximately from +300 to -700 mV vs the normal hydrogen electrode, which covers the range of most protein redox potentials accessible in aqueous solution. The complexes employed exhibit stability in both their oxidized and their reduced forms as well as pH-independent redox potentials within the range 6 < pH < 9. The mediators were also chosen on the basis of their very weak visible absorption maxima in both oxidation states, which will enable (for the first time) optical redox potentiometric titrations of proteins with relatively low extinction coefficients. This has previously been impractical with organic mediators, such as indoles, viologens and quinones, whose optical spectra interfere strongly with those of the protein.
Keyword Redox Potentiometry
Protein
Metal Complex
Biochemistry & Molecular Biology
Chemistry, Inorganic & Nuclear
Heart Cytochrome-c
Ruthenium-modified Proteins
Electron-transfer
Cobalt(iii)
Ligand
Reduction
Myoglobin
Cage
Electrochemistry
Oxidation
Q-Index Code C1

 
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Created: Wed, 15 Aug 2007, 19:26:04 EST