The inhibitory effects of free nitrous acid on the energy generation and growth processes of an enriched Nitrobacter culture

Vadivelu, V. M., Yuan, Z. G., Fux, C. and Keller, J. (2006) The inhibitory effects of free nitrous acid on the energy generation and growth processes of an enriched Nitrobacter culture. Environmental Science & Technology, 40 14: 4442-4448. doi:10.1021/es051694k


Author Vadivelu, V. M.
Yuan, Z. G.
Fux, C.
Keller, J.
Title The inhibitory effects of free nitrous acid on the energy generation and growth processes of an enriched Nitrobacter culture
Journal name Environmental Science & Technology   Check publisher's open access policy
ISSN 0013-936X
Publication date 2006-01-01
Year available 2006
Sub-type Article (original research)
DOI 10.1021/es051694k
Open Access Status Not yet assessed
Volume 40
Issue 14
Start page 4442
End page 4448
Total pages 7
Editor J. Schnoor
Place of publication Washington
Publisher Amer Chemical Society
Language eng
Subject C1
299999 Engineering and Technology not elsewhere classified
779999 Other
Abstract The inhibitory effects of nitrite (NO2-)/free nitrous acid (HNO2-FNA) on the metabolism of Nitrobacter were investigated using a method allowing the decoupling of the growth and energy generation processes. A lab-scale sequencing batch reactor was operated for the enrichment of a Nitrobacter culture. Fluorescent in situ hybridization (FISH) analysis showed that 73% of the bacterial population was Nitrobacter. Batch tests were carried out to assess the oxygen and nitrite consumption rates of the enriched culture at low and high nitrite levels, in the presence or absence of inorganic carbon. It was observed that in the absence of CO2, the Nitrobacter culture was able to oxidize nitrite at a rate that is 76% of that in the presence of CO2, with an oxygen consumption rate that is 85% of that measured in the presence of CO2. This enabled the impacts of nitrite/FNA on the catabolic and anabolic processes of Nitrobacter to be assessed separately. FNA rather than nitrite was likely the actual inhibitor to the Nitrobacter metabolism. It was revealed that FNA of up to 0.05 mg HNO2-N center dot L-1 (3.4 mu M), which was the highest FNA concentration used in this study, did not have any inhibitory effect on the catabolic processes of Nitrobacter. However, FNA initiated its inhibition to the anabolic processes of Nitrobacter at approximately 0.011 mg HNO2-N center dot L-1 (0.8 mu M), and completely stopped biomass synthesis at a concentration of approximately 0.023 mg HNO2-N center dot L-1 (1.6 mu M). The inhibitory effect could be described by an empirical inhibitory model proposed in this paper, but the underlying mechanisms remain to be revealed.
Keyword Engineering, Environmental
Environmental Sciences
Waste-water
Maintenance Energy
Treatment Plants
Removal
Denitrification
Nitrification
Bacteria
Nitrite
Sharon
Q-Index Code C1
Institutional Status UQ

 
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Created: Wed, 15 Aug 2007, 19:21:30 EST