Synaptic activation of transient receptor potential channels by metabotropic glutamate receptors in the lateral amygdala

Faber, E. S. L., Sedlak, P., Vidovic, M. and Sah, P. (2006) Synaptic activation of transient receptor potential channels by metabotropic glutamate receptors in the lateral amygdala. Neuroscience, 137 3: 781-794. doi:10.1016/j.neuroscience.2005.09.027


Author Faber, E. S. L.
Sedlak, P.
Vidovic, M.
Sah, P.
Title Synaptic activation of transient receptor potential channels by metabotropic glutamate receptors in the lateral amygdala
Journal name Neuroscience   Check publisher's open access policy
ISSN 0306-4522
Publication date 2006-01-01
Sub-type Article (original research)
DOI 10.1016/j.neuroscience.2005.09.027
Volume 137
Issue 3
Start page 781
End page 794
Total pages 14
Editor O. P. Ottersen
Place of publication Oxford, UK
Publisher Pergamon-Elsevier Science Ltd
Collection year 2006
Language eng
Subject C1
320702 Central Nervous System
730104 Nervous system and disorders
Abstract Classical mammalian transient receptor potential channels form non-selective cation channels that open in response to activation of phospholipase C-coupled metabotropic receptors, and are thought to play a key role in calcium homeostasis in non-excitable cells. Within the nervous system transient receptor potential channels are widely distributed but their physiological roles are not well understood. Here we show that in the rat lateral amygdala transient receptor potential channels mediate an excitatory synaptic response to glutamate. Activation of group l etabotropic glutamate receptors on pyramidal neurons in the lateral amygdala with either exogenous or synaptically released glutamate evokes an inward current at negative potentials with a current voltage relationship showing a region of negative slope and steep outward rectification. This current is blocked by inhibiting G protein function with GTP-beta-S, by inhibiting phospholipase C or by infusing transient receptor potential antibodies into lateral amygdala pyramidal neurons. Using RT-PCR and Western blotting we show that transient receptor potential 1, transient receptor potential 4 and transient receptor potential 5 are present in the lateral amygdala. Single cell PCR confirms the presence of transient receptor potential 1 and transient receptor potential 5 in pyramidal neurons and we show by co-immunoprecipitation that transient receptor potential 1 and transient receptor potential 5 co-assemble as a heteromultimers in the amygdala. These results show that in lateral amygdala pyramidal neurons synaptically released glutamate activates transient receptor potential channels, which we propose are likely to be heteromultimeric channels containing transient receptor potential 1 and transient receptor potential 5/transient receptor potential 4. (c) 2005 Published by Elsevier Ltd on behalf of IBRO.
Keyword Learning
Calcium Waves
Fear
Synaptic Plasticity
Ltp
Neurosciences
Nonselective Cation Channels
Ca1 Pyramidal Neurons
Electrophysiological Properties
Dopamine Neurons
Store Depletion
Messenger-rna
Trp Channel
Ca2+ Entry
Expression
Brain
Q-Index Code C1

 
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Created: Wed, 15 Aug 2007, 19:17:35 EST