Sugarcane ShSUT1: analysis of sucrose transport activity and inhibition by sucralose

Reinders, Anke, Sivitz, Alicia B., Hsi, Alex, Grof, Christopher P. L., Perroux, Jai M. and Ward, John M. (2006) Sugarcane ShSUT1: analysis of sucrose transport activity and inhibition by sucralose. Plant Cell and Environment, 29 10: 1871-1880. doi:10.1111/j.1365-3040.2006.01563.x

Author Reinders, Anke
Sivitz, Alicia B.
Hsi, Alex
Grof, Christopher P. L.
Perroux, Jai M.
Ward, John M.
Title Sugarcane ShSUT1: analysis of sucrose transport activity and inhibition by sucralose
Journal name Plant Cell and Environment   Check publisher's open access policy
ISSN 0140-7791
Publication date 2006-01-01
Sub-type Article (original research)
DOI 10.1111/j.1365-3040.2006.01563.x
Volume 29
Issue 10
Start page 1871
End page 1880
Total pages 10
Place of publication Oxford
Publisher Blackwell Publishing
Language eng
Subject C1
Abstract Plant sucrose transporters (SUTs) are members of the glycoside-pentoside-hexuronide (GPH) cation symporter family (TC2.A.2) that is part of the major facilitator superfamily (MFS). All plant SUTs characterized to date function as proton-coupled symporters and catalyze the cellular uptake of sucrose. SUTs are involved in loading sucrose into the phloem and sink tissues, such as seeds, roots and flowers. Because monocots are agriculturally important, SUTs from cereals have been the focus of recent research. Here we present a functional analysis of the SUT ShSUT1 from sugarcane, an important crop species grown for its ability to accumulate high amounts of sucrose in the stem. ShSUT1 was previously shown to be expressed in maturing stems and plays an important role in the accumulation of sucrose in this tissue. Using two-electrode voltage clamping in Xenopus oocytes expressing ShSUT1, we found that ShSUT1 is highly selective for sucrose, but has a relatively low affinity for sucrose (K-0.5 = 8.26 mM at pH 5.6 and a membrane potential of -137 mV). We also found that the sucrose analog sucralose (4,1 ',6 '-trichloro-4,1 ',6 '-trideoxygalactosucrose) is a competitive inhibitor of ShSUT1 with an inhibition coefficient (K-i) of 16.5 mM. The presented data contribute to our understanding of sucrose transport in plants in general and in monocots in particular.
Keyword cotransporter
Xenopus oocytes
Plant Sucrose
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2007 Higher Education Research Data Collection
School of Agriculture and Food Sciences
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Citation counts: TR Web of Science Citation Count  Cited 46 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 48 times in Scopus Article | Citations
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Created: Wed, 15 Aug 2007, 19:16:55 EST