Stoichiometric and kinetic characterisation of Nitrobacter in mixed culture by decoupling the growth and energy generation processes

Vadivelu, Vel M., Yuan, Zhiguo, Fux, Christian and Keller, Jurg (2006) Stoichiometric and kinetic characterisation of Nitrobacter in mixed culture by decoupling the growth and energy generation processes. Biotechnology And Bioengineering, 94 6: 1176-1188. doi:10.1002/bit.20956


Author Vadivelu, Vel M.
Yuan, Zhiguo
Fux, Christian
Keller, Jurg
Title Stoichiometric and kinetic characterisation of Nitrobacter in mixed culture by decoupling the growth and energy generation processes
Journal name Biotechnology And Bioengineering   Check publisher's open access policy
ISSN 0006-3592
Publication date 2006-01-01
Sub-type Article (original research)
DOI 10.1002/bit.20956
Open Access Status Not Open Access
Volume 94
Issue 6
Start page 1176
End page 1188
Total pages 13
Editor D.S. Clark
Place of publication USA
Publisher John Wiley & Sons Inc
Language eng
Subject C1
299999 Engineering and Technology not elsewhere classified
779999 Other
Abstract The growth, maintenance and lysis processes of Nitrobacter were characterised. A Nitrobacter culture was enriched in a sequencing batch reactor (SBR). Fluorescent in situ hybridisation showed that Nitrobacter constituted 73% of the bacterial population. Batch tests were carried out to measure the oxygen uptake rate and/or nitrite consumption rate when both nitrite and CO2 were in excess, and in the absence of either of these two substrates. The results obtained, along with the SBR performance data, allowed the determination of the maintenance coefficient and in situ cell lysis rate of Nitrobacter. Nitrobacter spends a significant amount of energy for maintenance, which varies considerably with the specific growth rate. At maximum growth, Nitrobacter consume nitrite at a rate of 0.042 mgN/mgCOD(biomass)center dot h for maintenance purposes, which increases more than threefold to 0.143 mgN/mgCOD(biomass)center dot h in the absence of growth. In the SBR, where Nitrobacter grew at 40% of its maximum growth rate, a maintenance coefficient of 0.113 mgN/mgCOD center dot h was found, resulting in 42% of the total amount of nitrite being consumed for maintenance. The above three maintenance coefficient values obtained at different growth rates appear to support the maintenance model proposed in Pirt (1982). The in situ lysis rate of Nitrobacter was determined to be 0.07/day under aerobic conditions at 22 C and pH 7.3. Further, the maximum specific growth rate of Nitrobacter was estimated to be 0.02/h (0.48/day). The affinity constant of Nitrobacter with respect to nitrite was determined to be 1.50 mgNO(2)(-)-N/L, independent of the presence or absence of CO2. (c) 2006 Wiley Periodicals, Inc.
Keyword Biotechnology & Applied Microbiology
Nitrobacter Maintenance Coefficient
Specific Growth Rate
In Situ Cell Lysis Rate
Waste-water Treatment
Off-gas Analysis
Maintenance Energy
Nitrifying Bacteria
Activated-sludge
Nitrosomonas-europaea
Nitrogen Removal
Treatment Plants
Biomass Decay
Nitrification
Q-Index Code C1

 
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Created: Wed, 15 Aug 2007, 19:16:04 EST