Real-time PCR assays for detection of bocavirus in human specimens

Lu, Xiaoyan, Chittaganpitch, Malinee, Olsen, Sonja J., Mackay, Ian M., Sloots, Theo P., Fry, Alicia M. and Erdman, Dean D. (2006) Real-time PCR assays for detection of bocavirus in human specimens. Journal Of Clinical Microbiology, 44 9: 3231-3235. doi:10.1128/JCM.00889-06

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Author Lu, Xiaoyan
Chittaganpitch, Malinee
Olsen, Sonja J.
Mackay, Ian M.
Sloots, Theo P.
Fry, Alicia M.
Erdman, Dean D.
Title Real-time PCR assays for detection of bocavirus in human specimens
Journal name Journal Of Clinical Microbiology   Check publisher's open access policy
ISSN 0095-1137
1098-660X
Publication date 2006-01-01
Sub-type Article (original research)
DOI 10.1128/JCM.00889-06
Open Access Status File (Publisher version)
Volume 44
Issue 9
Start page 3231
End page 3235
Total pages 5
Editor Andrew B Onderdonk Editor in Chief
Place of publication Washington, DC, United States
Publisher American Society Microbiology
Collection year 2006
Language eng
Abstract The recently discovered human bocavirus (HBoV) is the first member of the family Parpoviridae, genus Bocavirus, to be potentially associated with human disease. Several studies have identified HBoV in respiratory specimens from children with acute respiratory disease, but the full spectrum of clinical disease and the epidemiology of HBoV infection remain unclear. The availability of rapid and reliable molecular diagnostics would therefore aid future studies of this novel virus. To address this, we developed two sensitive and specific real-time TaqMan PCR assays that target the HBoV NS1 and NP-1 genes. Both assays could reproducibly detect 10 copies of a recombinant DNA plasmid containing a partial region of the HBoV genome, with a dynamic range of 8 log units (10(1) to 10(8) copies). Eight blinded clinical specimen extracts positive for HBoV by an independent PCR assay were positive by both real-time assays. Among 1,178 NP swabs collected from hospitalized pneumonia patients in Sa Kaeo Province, Thailand, 53 (4.5%) were reproducibly positive for HBoV by one or both targets. Our data confirm the possible association of HBoV infection with pneumonia and demonstrate the utility of these real-time PCR assays for HBoV detection.
Keyword Microbiology
Real Time PCR assays
Coronavirus
Respiratory specimens
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

 
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Created: Wed, 15 Aug 2007, 19:09:17 EST