Quantitative analysis and characterization of biofunctionalized fluorescent silica particles

Corrie, Simon R., Lawrie, Gwendolyn A. and Trau, Matt (2006) Quantitative analysis and characterization of biofunctionalized fluorescent silica particles. Langmuir, 22 6: 2731-2737. doi:10.1021/la052433r


Author Corrie, Simon R.
Lawrie, Gwendolyn A.
Trau, Matt
Title Quantitative analysis and characterization of biofunctionalized fluorescent silica particles
Journal name Langmuir   Check publisher's open access policy
ISSN 0743-7463
Publication date 2006-01-01
Sub-type Article (original research)
DOI 10.1021/la052433r
Open Access Status Not Open Access
Volume 22
Issue 6
Start page 2731
End page 2737
Total pages 7
Place of publication Washington
Publisher Amer Chemical Soc
Language eng
Subject CX
030301 Chemical Characterisation of Materials
Abstract A strategy for the production and subsequent characterization of biofunctionalized silica particles is presented. The particles were engineered to produce a bifunctional material capable of both (a) the attachment of fluorescent dyes for particle encoding and (b) the sequential modification of the surface of the particles to couple oligonucleotide probes. A combination of microscopic and analytical methods is implemented to demonstrate that modification of the particles with 3-aminopropyl trimethoxysilane results in an even distribution of amine groups across the particle surface. Evidence is provided to indicate that there are negligible interactions between the bound fluorescent dyes and the attached biomolecules. A unique approach was adopted to provide direct quantification of the oligonucleotide probe loading on the particle surface through X-ray photoelectron spectroscopy, a technique which may have a major impact for current researchers and users of bead-based technologies. A simple hybridization assay showing high sequence specificity is included to demonstrate the applicability of these particles to DNA screening.
Keyword Chemistry, Physical
Ray Photoelectron-spectroscopy
Combinatorial Libraries
Colloidal Silica
Surface
Dna
3-mercaptopropyltrimethoxysilane
Hybridization
Spheres
Xps
3-aminopropyltrimethoxysilane
Q-Index Code CX

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
Australian Institute for Bioengineering and Nanotechnology Publications
 
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Created: Wed, 15 Aug 2007, 19:08:24 EST