Identification of candidate genes at the corticoseptal boundary during development

Shen, Wei-Bin, Plachez, Celine, Mongi, Alka S. and Richards, Linda J. (2006) Identification of candidate genes at the corticoseptal boundary during development. Gene Expression Patterns, 6 5: 471-481. doi:10.1016/j.modgep.2005.11.004

Author Shen, Wei-Bin
Plachez, Celine
Mongi, Alka S.
Richards, Linda J.
Title Identification of candidate genes at the corticoseptal boundary during development
Journal name Gene Expression Patterns   Check publisher's open access policy
ISSN 1567-133X
Publication date 2006-06-01
Year available 2006
Sub-type Article (original research)
DOI 10.1016/j.modgep.2005.11.004
Open Access Status
Volume 6
Issue 5
Start page 471
End page 481
Total pages 11
Editor M. E. Halpern
S. Aizawa
Place of publication Amsterdam
Publisher Elsevier Science Bv
Language eng
Subject C1
270210 Neurogenetics
730104 Nervous system and disorders
Abstract Cortical midline glia are critical to the formation of the corpus callosum during development. The glial wedge is a Population of midline glia that is located at the corticoseptal boundary and expresses repulsive/growth-inhibitory molecules that guide callosal axons as they cross the midline. The glial wedge are the first cells within the cortex to express GFAP and thus may express molecules specific for glial maturation. The corticoseptal boundary is a genetically defined boundary between the cingulate cortex (dorsal telencephalon) and the septum (ventral telencephalon). The correct dorso-ventral position of this boundary is vital to the formation of both the glial wedge and the corpus callosum. Our aim was to identify genes expressed specifically within the glial wedge that might be involved in either glial differentiation, formation of the corticoseptal boundary or development of the corpus callosum. To identify such genes we have performed a differential display PCR screen comparing RNA isolated from the glial wedge with RNA isolated from control tissues such as the neocortex and septum, of embryonic day 17 mouse brains. Using 200 different combinations of primers, we identified and cloned 67 distinct gene fragments. In situ hybridization analysis confirmed the differential expression of many of the genes, and showed that clones G24F3, G39F8 and transcription factor LZIP have specific expression patterns in the telencephalon of embryonic and postnatal brains. An RNase Protection Assay (RPA) revealed that the expression of G39F8, G24173 and LZIP increase markedly in the telencephalon at E16 and continue to be expressed until at least PO, during the period when the corpus callosum is forming. (c) 2005 Elsevier B.V. All rights reserved.
Keyword Midline Glia
Glial Wedge
Axon Guidance
Differential Display Pcr
Zinc Finger Protein 288
Developmental Biology
Genetics & Heredity
Q-Index Code C1
Institutional Status UQ

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Created: Wed, 15 Aug 2007, 18:58:30 EST