Identification and analysis of novel genes expressed in the mouse embryonic facial primordia

Bennetts, Jennifer S., Fowles, Lindsay F., Butterfield, Natalie C., Berkman, Jennifer L., Teasdale, Rohan D., Simpson, Fiona and Wicking, Carol (2006) Identification and analysis of novel genes expressed in the mouse embryonic facial primordia. Frontiers In Bioscience, 11 2631-2646. doi:10.2741/1997

Author Bennetts, Jennifer S.
Fowles, Lindsay F.
Butterfield, Natalie C.
Berkman, Jennifer L.
Teasdale, Rohan D.
Simpson, Fiona
Wicking, Carol
Title Identification and analysis of novel genes expressed in the mouse embryonic facial primordia
Journal name Frontiers In Bioscience   Check publisher's open access policy
ISSN 1093-9946
Publication date 2006-01-01
Year available 2006
Sub-type Article (original research)
DOI 10.2741/1997
Open Access Status
Volume 11
Start page 2631
End page 2646
Total pages 16
Editor Siamak Tabibzadeh
Place of publication Manhasset
Publisher Frontiers In Bioscience Inc.
Language eng
Subject C1
780105 Biological sciences
270106 Cell Development (incl. Cell Division and Apoptosis)
Abstract Craniofacial anomalies are a common feature of human congenital dysmorphology syndromes, suggesting that genes expressed in the developing face are likely to play a wider role in embryonic development. To facilitate the identification of genes involved in embryogenesis, we previously constructed an enriched cDNA library by subtracting adult mouse liver cDNA from that of embryonic day (E)10.5 mouse pharyngeal arch cDNA. From this library, 273 unique clones were sequenced and known proteins binned into functional categories in order to assess enrichment of the library (1). We have now selected 31 novel and poorly characterised genes from this library and present bioinformatic analysis to predict proteins encoded by these genes, and to detect evolutionary conservation. Of these genes 61% (19/31) showed restricted expression in the developing embryo, and a subset of these was chosen for further in silico characterisation as well as experimental determination of subcellular localisation based on transient transfection of predicted full-length coding sequences into mammalian cell lines. Where a human orthologue of these genes was detected, chromosomal localisation was determined relative to known loci for human congenital disease.
Keyword Biochemistry & Molecular Biology
Cell Biology
Craniofacial Development
Pharyngeal Arches
Novel Genes
In Situ Hybridisation
Subcellular Localisation
Craniofacial Morphogenesis
Poly(a)-binding Protein
Functional Annotation
Subtelomeric Deletion
Multiprotein Complex
Transcription Factor
Molecular Signals
Q-Index Code C1
Institutional Status UQ

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Created: Wed, 15 Aug 2007, 18:58:13 EST