Definition and spatial annotation of the dynamic secretome during early kidney development

Martinez, Gemma, Georgas, Kylie, Challen, Grant A., Rumballe, Bree, Davis, Melissa J., Taylor, Darrin, Teasdale, Rohan D., Grimmond, Sean M. and Little, Melissa H. (2006) Definition and spatial annotation of the dynamic secretome during early kidney development. Developmental Dynamics, 235 6: 1709-1719. doi:10.1002/dvdy.20740

Author Martinez, Gemma
Georgas, Kylie
Challen, Grant A.
Rumballe, Bree
Davis, Melissa J.
Taylor, Darrin
Teasdale, Rohan D.
Grimmond, Sean M.
Little, Melissa H.
Title Definition and spatial annotation of the dynamic secretome during early kidney development
Journal name Developmental Dynamics   Check publisher's open access policy
ISSN 1058-8388
Publication date 2006-06-01
Year available 2006
Sub-type Article (original research)
DOI 10.1002/dvdy.20740
Open Access Status
Volume 235
Issue 6
Start page 1709
End page 1719
Total pages 11
Editor Gary C. Schoenwolf
Place of publication Hobroken, USA
Publisher John Wiley & Sons Inc.
Language eng
Subject C1
270205 Genetic Development (incl. Sex Determination)
780105 Biological sciences
Abstract The term secretome has been defined as a set of secreted proteins (Grimmond et al. [2003] Genome Res 13:1350-1359). The term secreted protein encompasses all proteins exported from the cell including growth factors, extracellular proteinases, morphogens, and extracellular matrix molecules. Defining the genes encoding secreted proteins that change in expression during organogenesis, the dynamic secretome, is likely to point to key drivers of morphogenesis. Such secreted proteins are involved in the reciprocal interactions between the ureteric bud (UB) and the metanephric mesenchyme (AM) that occur during organogenesis of the metanephros. Some key metanephric secreted proteins have been identified, but many remain to be determined. In this study, microarray expression profiling of E10.5, E11.5, and E13.5 kidney and consensus bioinformatic analysis were used to define a dynamic secretome of early metanephric development. In situ hybridisation was used to confirm microarray results and clarify spatial expression patterns for these genes. Forty-one secreted factors were dynamically expressed between the E10.5 and E13.5 timeframe profiled, and 25 of these factors had not previously been implicated in kidney development. A text-based anatomical ontology was used to spatially annotate the expression pattern of these genes in cultured metanephric explants.
Keyword Kidney Development
Expression Profiling
Explant Culture
Anatomy & Morphology
Developmental Biology
Gene-expression Patterns
Ureteral Bud
Metanephric Mesenchyme
Restricted Expression
Q-Index Code C1
Grant ID DK63400
Institutional Status UQ

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Created: Wed, 15 Aug 2007, 18:35:55 EST