Optimisation of RNA Extraction from Gracilaria changii (Gracilariales, Rhodophyta)

Chan, Cheong Xin, Teo, Swee-Sen, Ho, Chai-Ling, Othman, Rofina Yasmin and Phang, Siew-Moi (2004) Optimisation of RNA Extraction from Gracilaria changii (Gracilariales, Rhodophyta). Journal of Applied Phycology, 16 4: 297-301. doi:10.1023/B:JAPH.0000047782.20940.de

Attached Files (Some files may be inaccessible until you login with your UQ eSpace credentials)
Name Description MIMEType Size Downloads
Read with bookreader  Chan_CX_et_al.JA.pdf Chan_CX_et_al.JA.pdf application/pdf 70.73KB 0

Author Chan, Cheong Xin
Teo, Swee-Sen
Ho, Chai-Ling
Othman, Rofina Yasmin
Phang, Siew-Moi
Title Optimisation of RNA Extraction from Gracilaria changii (Gracilariales, Rhodophyta)
Journal name Journal of Applied Phycology   Check publisher's open access policy
ISSN 0921-8971
Publication date 2004-08-01
Sub-type Article (original research)
DOI 10.1023/B:JAPH.0000047782.20940.de
Open Access Status File (Author Post-print)
Volume 16
Issue 4
Start page 297
End page 301
Total pages 5
Language eng
Subject 270804 Genetic Technologies - Transformation, Site-directed Mutagenesis, etc.
270404 Phycology
270199 Biochemistry and Cell Biology not elsewhere classified
Abstract RNA extraction from seaweed tissues is problematic due to the presence of polysaccharides and polyphenolic compounds upon cell disruption. Besides, a successful RNA isolation from seaweed tissues can sometimes be strain- and species-specific. Four different methods were used to extract RNA from Gracilaria changii (Gracilariales, Rhodophyta), collected from the mangrove area at Morib, Selangor, Malaysia. An optimised and modified total RNA extraction method was developed for this recalcitrant species. The use of sand in tissue grinding, and the incorporation of phenol extraction at the initial stage resulted in the highest RNA yield (0.65-1.14 microg.g^-1 fresh weight) with high quality (A260:280 ratio 1.80-2.05). The RNA obtained is suitable for cDNA synthesis and future functional genomic studies.
Keyword Gracilaria changii
Malaysia
polysaccharides
RNA extraction
seaweed
References Ainsworth C (1994) Isolation of RNA from floral tissue of Rumex acetosa (Sorrel). Pl. Mol. Biol. Reptr. 12: 198-203. Apt KE, Clendennen SK, Powers DA, Grossman AR (1995) The gene family encoding the fucoxanthin chlorophyll proteins from the brown alga Macrocystis pyrifera. Mol. Gen. Genet. 246: 455-464. Dring MJ (1982) The Biology of Marine Plants. Cambridge University Press, Cambridge, pp. 199. Evans LV (1963) The use of lithium chloride as a pretreatment to an acetocarmine technique on Fucus. Phycologia 2: 187-195. Gehrig HH, Winter K, Cushman J, Borland A, Taybi T (2000) An improved RNA isolation method for succulent plant species rich in polyphenols and polysaccharides. Pl. Mol. Biol. Reptr. 18: 369-376. Guena F, Hartings H, Scienza A (1998) A new method for rapid extraction of high quality RNA from recalcitrant tissues of grapevine. Pl. Mol. Biol. Reptr. 16: 61-67. Gliscaronin V, Crkvenjakov R, Byus C (1974) Ribonucleic acid iso-lated by cesium chloride centrifugation. Biochemistry 13: 2633-2637. Ho CL, Phang SM, Sinnappah ND, Pang T (1996) Molecular ap-proaches in the taxonomy of the red and brown seaweeds. In Chaudhary BR, Agrawal SB (eds), Cytolology, Genetics andMolecular Biology of Algae. SPB Academic Publishing, Am-sterdam, pp. 351-362. Hong YK, Kim SD, Polne-Fuller M, Gibor A(1995) DNAextraction conditions from Porphyra perforata using LiCl. J. Appl. Phycol. 7: 101-107. Hong YK, Sohn CH, Lee KW, Kim HG (1997) Nucleic acid extrac-tion from seaweed tissues for polymerase chain reaction. J. Mar. Biotechnol. 5: 95-99. Kim SH, Lee YK, Hong CB, Lee IK (1997) A simple method for RNA extraction from marine macroalgae. Algae 12: 53-56. Lim PE, Thong KL, Phang SM (2001) Molecular differentiation of two morphological variants of Gracilaria salicornia. J.Appl. Phycol. 13: 335-342. Liu JJ, Goh CJ, Loh CS, Liu P, Pua EC (1998) A method for isolation of total RNA from fruit tissues of banana. Pl. Mol. Biol. Reptr. 16: 1-6. Norziah MH, Ching CY (2000) Nutritional composition of edible seaweed Gracilaria changii. Food Chem. 68: 69-76. Phang SM (1994) Some species of Gracilaria from Peninsular Malaysia and Singapore. In Abbott IA (ed), Taxonomy of Eco-nomic Seaweeds Vol. IV. California Sea Grant Programme, Uni-versity of California, California, pp.125-134. Phang SM, Shaharuddin S, Noraishah H, Sasekumar A (1996) Studies on Gracilaria changii (Gracilariales, Rhodophyta) from Malaysian mangroves. Hydrobiologia 326/327: 347-352.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown
Additional Notes This is an author version of an article originally published as Cheong-Xin Chan, Swee-Sen Teo, Chai-Ling Ho, Rofina Yasmin Othman and Siew-Moi Phang (2004) Optimisation of RNA extraction from Gracilaria changii (Gracilariales, Rhodophyta) Journal of Applied Phycology 16 (4)297-301. DOI 10.1023/B:JAPH.0000047782.20940.de Copyright 2004 Springer-Verlag. All rights reserved. The original publication is available at www.springerlink.com.

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
Institute for Molecular Bioscience - Publications
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 29 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 33 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Mon, 04 Sep 2006, 10:00:00 EST by Cheong Xin Chan on behalf of Institute for Molecular Bioscience