Optimisation of RNA Extraction from Gracilaria changii (Gracilariales, Rhodophyta)

Chan, Cheong Xin, Teo, Swee-Sen, Ho, Chai-Ling, Othman, Rofina Yasmin and Phang, Siew-Moi (2004) Optimisation of RNA Extraction from Gracilaria changii (Gracilariales, Rhodophyta). Journal of Applied Phycology, 16 4: 297-301. doi:10.1023/B:JAPH.0000047782.20940.de

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Author Chan, Cheong Xin
Teo, Swee-Sen
Ho, Chai-Ling
Othman, Rofina Yasmin
Phang, Siew-Moi
Title Optimisation of RNA Extraction from Gracilaria changii (Gracilariales, Rhodophyta)
Journal name Journal of Applied Phycology   Check publisher's open access policy
ISSN 0921-8971
Publication date 2004-08-01
Sub-type Article (original research)
DOI 10.1023/B:JAPH.0000047782.20940.de
Open Access Status File (Author Post-print)
Volume 16
Issue 4
Start page 297
End page 301
Total pages 5
Language eng
Subject 270804 Genetic Technologies - Transformation, Site-directed Mutagenesis, etc.
270404 Phycology
270199 Biochemistry and Cell Biology not elsewhere classified
Abstract RNA extraction from seaweed tissues is problematic due to the presence of polysaccharides and polyphenolic compounds upon cell disruption. Besides, a successful RNA isolation from seaweed tissues can sometimes be strain- and species-specific. Four different methods were used to extract RNA from Gracilaria changii (Gracilariales, Rhodophyta), collected from the mangrove area at Morib, Selangor, Malaysia. An optimised and modified total RNA extraction method was developed for this recalcitrant species. The use of sand in tissue grinding, and the incorporation of phenol extraction at the initial stage resulted in the highest RNA yield (0.65-1.14 microg.g^-1 fresh weight) with high quality (A260:280 ratio 1.80-2.05). The RNA obtained is suitable for cDNA synthesis and future functional genomic studies.
Keyword Gracilaria changii
Malaysia
polysaccharides
RNA extraction
seaweed
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown
Additional Notes This is an author version of an article originally published as Cheong-Xin Chan, Swee-Sen Teo, Chai-Ling Ho, Rofina Yasmin Othman and Siew-Moi Phang (2004) Optimisation of RNA extraction from Gracilaria changii (Gracilariales, Rhodophyta) Journal of Applied Phycology 16 (4)297-301. DOI 10.1023/B:JAPH.0000047782.20940.de Copyright 2004 Springer-Verlag. All rights reserved. The original publication is available at www.springerlink.com.

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
Institute for Molecular Bioscience - Publications
 
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Citation counts: TR Web of Science Citation Count  Cited 29 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 34 times in Scopus Article | Citations
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Created: Mon, 04 Sep 2006, 10:00:00 EST by Cheong Xin Chan on behalf of Institute for Molecular Bioscience