Total RNA Extraction for the Red Seaweed Gracilaria changii (Gracilariales, Rhodophyta)

Chan, Cheong Xin, Ho, Chai-Ling, Othman, Rofina Yasmin and Phang, Siew-Moi (2002). Total RNA Extraction for the Red Seaweed Gracilaria changii (Gracilariales, Rhodophyta). In: Asia-Pacific Conference in Marine Science and Technology, Kuala Lumpur, Malaysia, (). 12-16 May, 2002.

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Author Chan, Cheong Xin
Ho, Chai-Ling
Othman, Rofina Yasmin
Phang, Siew-Moi
Title of paper Total RNA Extraction for the Red Seaweed Gracilaria changii (Gracilariales, Rhodophyta)
Conference name Asia-Pacific Conference in Marine Science and Technology
Conference location Kuala Lumpur, Malaysia
Conference dates 12-16 May, 2002
Publication Year 2002
Sub-type Fully published paper
Abstract/Summary Five different RNA extraction methods have been tried out on the red seaweed, Gracilaria changii collected from the mangrove area at Morib, Selangor, Malaysia. Two methods, one utilising guanidinium thiocyanate, and another using cetyltrimethyl ammonium bromide (CTAB), were found to be potential alternatives to obtain pure RNA. By incorporating sand while grinding the tissue, the method using CTAB was found most suitable to obtain pure RNA (high A260:280nm ratio) with high yield (0.16ug RNA per gram of fresh tissue).
Subjects 270804 Genetic Technologies - Transformation, Site-directed Mutagenesis, etc.
270404 Phycology
270199 Biochemistry and Cell Biology not elsewhere classified
Keyword RNA extraction
marine macroalgae
Gracilaria changii
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Q-Index Code E1
Q-Index Status Provisional Code
Institutional Status Unknown
Additional Notes This paper is later published as: Chan CX, Ho CL, Othman RY and Phang SM (2004). Optimisation of RNA extraction for Gracilaria changii (Gracilariales, Rhodophyta). In: Phang SM, Chong VC, Ho SC, Mokhtar N and Ooi JLS (eds.) Marine Science into the New Millenium: New Perspectives & Challenges. Kuala Lumpur: University of Malaya Maritime Research Centre. pp. 477-487.

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Created: Mon, 04 Sep 2006, 10:00:00 EST by Cheong Xin Chan