Black Sigatoka disease: new technologies to strengthen eradication strategies in Australia

Henderson, J., Pattemore, J. A., Porchun, S. C., Hayden, H. L., Van Brunschot, S., Grice, K. R. E., Peterson, R. A., Thomas-Hall, S. R. and Aitken, E. A. B. (2006) Black Sigatoka disease: new technologies to strengthen eradication strategies in Australia. Australasian Plant Pathology, 35 2: 181-193. doi:10.1071/AP06017


Author Henderson, J.
Pattemore, J. A.
Porchun, S. C.
Hayden, H. L.
Van Brunschot, S.
Grice, K. R. E.
Peterson, R. A.
Thomas-Hall, S. R.
Aitken, E. A. B.
Title Black Sigatoka disease: new technologies to strengthen eradication strategies in Australia
Journal name Australasian Plant Pathology   Check publisher's open access policy
ISSN 0815-3191
1448-6032
Publication date 2006-01-01
Sub-type Article (original research)
DOI 10.1071/AP06017
Volume 35
Issue 2
Start page 181
End page 193
Total pages 13
Place of publication Collingwood, Vic.
Publisher CSIRO
Language eng
Subject C1
620205 Tropical fruit
060704 Plant Pathology
Formatted abstract
In 2001, an incursion of Mycosphaerella fijiensis, the causal agent of black Sigatoka, was detected in Australia's largest commercial banana growing region, the Tully Banana Production Area in North Queensland. An intensive surveillance and eradication campaign was undertaken which resulted in the reinstatement of the disease-free status for black Sigatoka in 2005. This was the first time black Sigatoka had ever been eradicated from commercial plantations. The success of the eradication campaign was testament to good working relationships between scientists, growers, crop monitors, quarantine regulatory bodies and industry. A key contributing factor to the success was the deployment of a PCR-based molecular diagnostic assay, developed by the Cooperative Research Centre for Tropical Plant Protection (CRCTPP). This assay complemented morphological identification and allowed high throughput diagnosis of samples facilitating rapid decision-making during the eradication campaign. This paper describes the development and successful deployment of molecular diagnostics for black Sigatoka. Shortcomings in the gel-based assay are discussed and the advantages of highly specific real-time PCR assays, capable of differentiating between Mycosphaerella fijiensis, Mycosphaerella musicola and Mycosphaerella eumusae are outlined. Real-time assays may provide a powerful diagnostic tool for applications in surveillance, disease forecasting and resistance testing for Sigatoka leaf spot diseases.
Keyword Plant Sciences
Bananas Mycosphaerella-fijiensis
Leaf Streak Disease
Genetic-structure
Var Difformis
Causal Agent
Continental-scale
Pcr
Musicola
Spot
Quantification
Q-Index Code C1

 
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Created: Wed, 15 Aug 2007, 18:28:30 EST