Hypoxic/ischemic models in newborn piglet: Comparison of constant FiO(2) versus variable FiO(2) delivery

Bjorkman, S. Tracey, Foster, Kelly A., O'Driscoll, Stephanie M., Healy, Genevieve N., Lingwood, Barbara E., Burke, Chris and Colditz, Paul B. (2006) Hypoxic/ischemic models in newborn piglet: Comparison of constant FiO(2) versus variable FiO(2) delivery. Brain Research, 1100 1: 110-117. doi:10.1016/j.brainres.2006.04.119

Author Bjorkman, S. Tracey
Foster, Kelly A.
O'Driscoll, Stephanie M.
Healy, Genevieve N.
Lingwood, Barbara E.
Burke, Chris
Colditz, Paul B.
Title Hypoxic/ischemic models in newborn piglet: Comparison of constant FiO(2) versus variable FiO(2) delivery
Journal name Brain Research   Check publisher's open access policy
ISSN 0006-8993
Publication date 2006-01-01
Year available 2006
Sub-type Article (original research)
DOI 10.1016/j.brainres.2006.04.119
Open Access Status Not yet assessed
Volume 1100
Issue 1
Start page 110
End page 117
Total pages 8
Editor F. E. Bloom
Place of publication Amsterdam, Netherlands
Publisher Elsevier BV
Language eng
Subject C1
320702 Central Nervous System
730204 Child health
Abstract A comparison of a constant (continuous delivery of 4% FiO(2)) and a variable (initial 5% FiO(2) with adjustments to induce low amplitude EEG (LAEEG) and hypotension) hypoxic/ischemic insult was performed to determine which insult was more effective in producing a consistent degree of survivable neuropathological damage in a newborn piglet model of perinatal asphyxia. We also examined which physiological responses contributed to this outcome. Thirty-nine 1-day-old piglets were subjected to either a constant hypoxic/ischemic insult of 30- to 37-min duration or a variable hypoxic/ischemic insult of 30-min low peak amplitude EEG (LAEEG < 5 mu V) including 10 min of low mean arterial blood pressure (MABP < 70% of baseline). Control animals (n = 6) received 21% FiO(2) for the duration of the experiment. At 72 h, the piglets were euthanased, their brains removed and fixed in 4% paraformaldehyde and assessed for hypoxic/ischemic injury by histological analysis. Based on neuropathology scores, piglets were grouped as undamaged or damaged; piglets that did not survive to 72 h were grouped separately as dead. The variable insult resulted in a greater number of piglets with neuropathological damage (undamaged = 12.5%, damaged = 68.75%, dead = 18.75%) while the constant insult resulted in a large proportion of undamaged piglets (undamaged = 50%, damaged = 22.2%, dead = 27.8%). A hypoxic insult varied to maintain peak amplitude EEG < 5 mu V results in a greater number of survivors with a consistent degree of neuropathological damage than a constant hypoxic insult. Physiological variables MABP, LAEEG, pH and arterial base excess were found to be significantly associated with neuropathological outcome. (c) 2006 Elsevier B.V. All rights reserved.
Keyword Hypoxia/ischemia
Newborn Piglet
Incomplete Cerebral-ischemia
Survival Model
Q-Index Code C1
Institutional Status UQ
Additional Notes This is a published research report

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Created: Wed, 15 Aug 2007, 18:23:20 EST