Heterotrimeric G proteins facilitate Arabidopsis resistance to necrotrophic pathogens and are involved in jasmonate signaling

Trusov, Y., Rookes, J. E., Chakravorty, D., Armour, D., Schenk, P. M. and Botella, J. R. (2006) Heterotrimeric G proteins facilitate Arabidopsis resistance to necrotrophic pathogens and are involved in jasmonate signaling. Plant Physiology, 140 1: 210-220. doi:10.1104/pp.105.069625


Author Trusov, Y.
Rookes, J. E.
Chakravorty, D.
Armour, D.
Schenk, P. M.
Botella, J. R.
Title Heterotrimeric G proteins facilitate Arabidopsis resistance to necrotrophic pathogens and are involved in jasmonate signaling
Journal name Plant Physiology   Check publisher's open access policy
ISSN 0032-0889
Publication date 2006-01-01
Year available 2006
Sub-type Article (original research)
DOI 10.1104/pp.105.069625
Open Access Status Not yet assessed
Volume 140
Issue 1
Start page 210
End page 220
Total pages 11
Place of publication Rockville
Publisher American Society of Plant Biologists
Language eng
Subject C1
270403 Plant Pathology
620101 Wheat
Abstract Heterotrimeric G proteinshave been previously linked to plant defense; however a role for the G beta gamma dimer in defense signaling has not been described to date. Using available Arabidopsis (Arabidopsis thaliana) mutants lacking functional G alpha or G beta subunits, we show that defense against the necrotrophic pathogens Alternaria brassicicola and Fusarium oxysporum is impaired in G beta- deficient mutants while G alpha-deficient mutants show slightly increased resistance compared to wild-type Columbia ecotype plants. In contrast, responses to virulent (DC3000) and avirulent (JL1065) strains of Pseudomonas syringae appear to be independent of heterotrimeric G proteins. The induction of a number of defense-related genes in G beta-deficient mutants were severely reduced in response to A. brassicicola infection. In addition, G beta-deficient mutants exhibit decreased sensitivity to a number of methyl jasmonate- induced responses such as induction of the plant defensin gene PDF1.2, inhibition of root elongation, seed germination, and growth of plants in sublethal concentrations of methyl jasmonate. In all cases, the behavior of the G alpha- deficient mutants is coherent with the classic heterotrimeric mechanism of action, indicating that jasmonic acid signaling is influenced by the Gbg functional subunit but not by G alpha. We hypothesize that G beta gamma acts as a direct or indirect enhancer of the jasmonate signaling pathway in plants.
Keyword Plant Sciences
Plant Defense Response
Gtp-binding Proteins
Oxidative Burst
Plasma-membrane
Alpha-subunit
Alternaria-brassicicola
Methyl Jasmonate
Abscisic-acid
Beta-subunit
Cell-death
Q-Index Code C1
Institutional Status UQ

 
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Created: Wed, 15 Aug 2007, 18:22:49 EST