Differential gene expression in the developing mouse ureter

Mitchell, E. K. L., Taylor, D. F., Woods, K., Davis, M. J., Nelson, A. L., Teasdale, R. D., Grimmond, S. M., Little, M. H., Bertram, J. F. and Caruana, G. (2006) Differential gene expression in the developing mouse ureter. Gene Expression Patterns, 6 5: 519-538. doi:10.1016/j.modgep.2005.10.008

Author Mitchell, E. K. L.
Taylor, D. F.
Woods, K.
Davis, M. J.
Nelson, A. L.
Teasdale, R. D.
Grimmond, S. M.
Little, M. H.
Bertram, J. F.
Caruana, G.
Title Differential gene expression in the developing mouse ureter
Journal name Gene Expression Patterns   Check publisher's open access policy
ISSN 1567-133X
Publication date 2006-01-01
Year available 2006
Sub-type Article (original research)
DOI 10.1016/j.modgep.2005.10.008
Open Access Status Not yet assessed
Volume 6
Issue 5
Start page 519
End page 538
Total pages 20
Editor S. Aizawa
M. Leptin
N. Papalopulu
D. Stainier
C.D. Stern
P. Tam
Place of publication Amsterdam
Publisher Elsevier Science
Language eng
Subject C1
270205 Genetic Development (incl. Sex Determination)
780105 Biological sciences
Abstract In many instances, kidney dysgenesis results as a secondary consequence to defects in the development of the ureter. Through the use of mouse genetics a number of genes associated with such malformations have been identified, however, the cause of many other abnormalities remain unknown. In order to identify novel genes involved in ureter development we compared gene expression in embryonic day (E) 12.5, E15.5 and postnatal day (P) 75 ureters using the Compugen mouse long oligo microarrays. A total of 248 genes were dynamically upregulated and 208 downregulated between E12.5 and P75. At E12.5, when the mouse ureter is comprised of a simple cuboidal epithelium surrounded by ureteric mesenchyme, genes previously reported to be expressed in the ureteric mesenchyme, foxC1 and foxC2 were upregulated. By E15.5 the epithelial layer develops into urothelium, impermeable to urine, and smooth muscle develops for the peristaltic movement of urine towards the bladder. The development of these two cell types coincided with the upregulation of UPIIIa, RAB27b and PPAR gamma reported to be expressed in the urothelium, and several muscle genes, Acta1, Tnnt2, Myocd, and Tpm2. In situ hybridization identified several novel genes with spatial expression within the smooth muscle, Acta1; ureteric mesenchyme and smooth muscle, Thbs2 and Co15a2; and urothelium, Kcnj8 and Adh1. This study marks the first known report defining global gene expression of the developing mouse ureter and will provide insight into the molecular mechanisms underlying kidney and lower urinary tract malformations. (c) 2005 Elsevier B.V. All rights reserved.
Keyword Ureter
Urinary Tract
Smooth Muscle
Uroplakin 3a (upiiia)
Angiotensin Ii Type 2 Receptor (agtr2)
Alcohol Dehydrogenase 1 (adh 1)
Actin Alpha 1 Skeletal Muscle (acta 1)
Potassium Inwardly-rectifyin
Developmental Biology
Genetics & Heredity
Potassium Inwardly-rectifying Channel Subfamily J Member 8 (kcnj8)
Thrombospondin 2 (thbs2)
Type V
Alpha 2 (col5a2)
Forkhead Box C1 (foxc1)
Forkhead Box C2 (foxc2)
Peroxisome Proliferator Activated Receptor Gamma (ppar Gamma)
Troponin T2
Cardiac (tnnt2)
Myocardin (myocd)
Tropomyosin 2
Beta (tpm2)
Mice Lacking Gdnf
Q-Index Code C1
Grant ID DK63400
Institutional Status UQ

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Created: Wed, 15 Aug 2007, 18:20:54 EST