Properties of a unique mutant of Helicoverpa armigera single-nucleocapsid nucleopolyhedrovirus that exhibits a partial many polyhedra and few polyhedra phenotype on extended serial passaging in suspension cell cultures

Pedrini, Marcia R. S., Nielsen, Lars K., Reid, Steven and Chan, Leslie C. L. (2005) Properties of a unique mutant of Helicoverpa armigera single-nucleocapsid nucleopolyhedrovirus that exhibits a partial many polyhedra and few polyhedra phenotype on extended serial passaging in suspension cell cultures. In Vitro Cellular & Developmental Biology - Animal, 41 8-9: 289-297. doi:10.1290/0503019R.1


Author Pedrini, Marcia R. S.
Nielsen, Lars K.
Reid, Steven
Chan, Leslie C. L.
Title Properties of a unique mutant of Helicoverpa armigera single-nucleocapsid nucleopolyhedrovirus that exhibits a partial many polyhedra and few polyhedra phenotype on extended serial passaging in suspension cell cultures
Journal name In Vitro Cellular & Developmental Biology - Animal   Check publisher's open access policy
ISSN 1071-2690
Publication date 2005-01-01
Year available 2005
Sub-type Article (original research)
DOI 10.1290/0503019R.1
Open Access Status Not yet assessed
Volume 41
Issue 8-9
Start page 289
End page 297
Total pages 9
Editor J. Denry Sato
Place of publication Largo, Maryland, USA
Publisher Society for In Vitro Biology
Language eng
Subject C1
270303 Virology
770804 Control of pests and exotic species
290101 Fermentation, Biotechnology and Industrial Microbiology
Abstract Serial passaging of wild-type Helicoverpa armigera, single-nucleocapsid (HaSNPV) in H. zea (HzAMI) illsect Cell Cultures results ill rapid selection for the few polyhedra (FP) phenotype. A unique HaSNPV mutant (ppC19) was isolated through plaque purification that exhibited a partial many polyhedra (MP) and FP phenotype. Oil serial passaging in suspension cell cultures, ppC19 produced fivefold more polyhedra than a typical FP mutant (FP8AS) but threefold less polyhedra than the wild-type virus. Most importantly, the polyhedra of ppC19 exhibited MP-like virion occlusion. Furthermore, ppC19 produced the same amount of budded virus (BV) as the FP mutant, which was fivefold higher than that of the wild-type virus. This selective advantage was likely to explain its relative stability in polyhedra production for six passages when compared with the wild-type Virus. However, subsequent passaging of ppC19 resulted in a steel) decline in both BV and polyhedra yields, which was also experienced by FP8AS and the wild-type virus Lit high passage numbers. Genomic deoxyribonueleic Licid profiling of the latter suggested that defective interfering particles (DIPS) were implicated in this phenomenon and represented another Undesirable mutation during serial passaging of HaSNPV Hence, a strategy to isolate HaSNPV Clones that exhibited MP-like polyhedra production but FP-like BV production, coupled with low multiplicities of infection during scale-up to avoid accumulation of DIPS, could prove commerically invaluable.
Keyword Cell Biology
Developmental Biology
Insect Cells
Hasnpv
Plaque Purification
Defective Interfering Particles
Fp Plaque Variants
Autographa-californica
Alfalfa Looper
Virus
Baculovirus
Replication
Genomes
Infectivity
Heliothis
Q-Index Code C1
Institutional Status UQ

 
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Created: Wed, 15 Aug 2007, 17:48:37 EST