Using urea dilution to standardise components of pleural and bronchoalveolar lavage fluids in the dog

Mills, PC and Litster, AL (2005) Using urea dilution to standardise components of pleural and bronchoalveolar lavage fluids in the dog. New Zealand Veterinary Journal, 53 6: 423-427. doi:10.1080/00480169.2005.36587


Author Mills, PC
Litster, AL
Title Using urea dilution to standardise components of pleural and bronchoalveolar lavage fluids in the dog
Journal name New Zealand Veterinary Journal   Check publisher's open access policy
ISSN 0048-0169
Publication date 2005-01-01
Sub-type Article (original research)
DOI 10.1080/00480169.2005.36587
Volume 53
Issue 6
Start page 423
End page 427
Total pages 5
Editor Peter Jolly
Place of publication New Zealand
Publisher NZ Vet Assoc.
Language eng
Subject C1
300512 Pharmacology
780105 Biological sciences
Abstract AIM: To develop a technique to estimate the volume of epithelial lining fluid (ELF) obtained during bronchoalveolar lavage (BAL) and pleural lavage (PL) in the dog, using the urea dilution method. METHODS: BAL and PL fluids were obtained by saline lavage of pulmonary and pleural cavities of nine clinically healthy mixed-breed dogs immediately after euthanasia. Cell counts in the BAL and PL fluids were measured using standard techniques. The concentration of ELF in each lavage fluid was calculated from the relative concentration of urea in plasma and in each type of lavage fluid. Cell counts in ELF were then calculated. RESULTS: There were substantially higher cell counts in ELF compared to BAL or PF fluid. However, nucleated cell counts in ELF could not be predicted from cell counts in BAL or PL fluid. CONCLUSIONS AND CLINICAL RELEVANCE: These results suggest that accurate assessment of cellular or non-cellular components in lavage fluids should include a calculation of the proportion of ELF recovered, using a method such as urea dilution.
Keyword Urea
Dilution
Pleural
Bronchoalveolar
Fluids
Dogs
Veterinary Sciences
Dog
Bronchoalveolar Lavage
Pleural Lavage
Epithelial Lining Fluid
Lactate-dehydrogenase
Peritoneal Effusions
Volume
Cats
Disease
Cells
Lung
Q-Index Code C1

 
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Created: Wed, 15 Aug 2007, 17:17:43 EST