Histopathology of oligofructose-induced acute laminitis in heifers

Thoefner, M. B., Wattle, O., Pollitt, C. C., French, K. R. and Nielsen, S. S. (2005) Histopathology of oligofructose-induced acute laminitis in heifers. Journal of Dairy Science, 88 8: 2774-2782. doi:10.3168/jds.S0022-0302(05)72957-X


Author Thoefner, M. B.
Wattle, O.
Pollitt, C. C.
French, K. R.
Nielsen, S. S.
Title Histopathology of oligofructose-induced acute laminitis in heifers
Journal name Journal of Dairy Science   Check publisher's open access policy
ISSN 0022-0302
1525-3198
Publication date 2005-08-01
Sub-type Article (original research)
DOI 10.3168/jds.S0022-0302(05)72957-X
Open Access Status
Volume 88
Issue 8
Start page 2774
End page 2782
Total pages 9
Editor Stephen C. Nickerson
Place of publication New York, NY, United States
Publisher Elsevier
Language eng
Subject 300506 Pathology
630107 Minor livestock (e.g. horses, goats, deer)
Abstract Histopathology of the dermo-epidermal junction in the lamellar region of front claws was examined in 6 dairy heifers given an alimentary oligofructose overload and compared with sections from a control group of 6 heifers. Four of the 6 heifers administered oligofructose developed clinical signs of acute laminitis before they were euthanized. Postmortem samples from front claws were processed for histology. Eleven histopathologic characteristics were selected from the existing literature and used in a blinded evaluation of sections. In total, 104 front claw samples, including 8 samples from 2 cows having spontaneously occurring acute laminitis, were evaluated histologically using hematoxylin and eosin as well as periodic acid-Schiff staining. The major morphological features associated with oligofructose-induced acute clinical laminitis were stretching of lamellae, dermal edema, hemorrhage, changes in basal cell morphology, presence of white blood cells in dermis, and signs of basement membrane detachment. Changes at the lamellar junction of claw tissue affected by oligofructose-induced clinical laminitis resembled tissue from the 2 cows suffering from spontaneous acute clinical laminitis, and generally were consistent with existing descriptions of laminitis histopathology. Important exceptions to existing descriptions in the literature were stretching of lamellae and basement membrane changes. Not previously described, we considered these early signs of acute laminitis. In conclusion, this study documents that oligofructose-induced clinical laminitis is associated with histopathological changes at the lamellar interface. A weakened dermo-epidermal junction is a possible intermediate stage in the pathophysiology of bovine sole ulceration at the typical site.
Keyword Bovine
Histopathology
Heifers
Laminitis
Oligofructose
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

 
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Created: Wed, 15 Aug 2007, 17:10:16 EST