Correlation of non-uniform protein expression with variation in transmitter release probability

Knight, David, Mann, Dwayne L., Jackson, V. Margaret, Trout, Stephen J., Cunnane, Tom C. and Lavidis, Nickolas A. (2005) Correlation of non-uniform protein expression with variation in transmitter release probability. Synapse, 55 2: 110-121. doi:10.1002/syn.20079

Author Knight, David
Mann, Dwayne L.
Jackson, V. Margaret
Trout, Stephen J.
Cunnane, Tom C.
Lavidis, Nickolas A.
Title Correlation of non-uniform protein expression with variation in transmitter release probability
Journal name Synapse   Check publisher's open access policy
ISSN 0887-4476
Publication date 2005-01-01
Sub-type Article (original research)
DOI 10.1002/syn.20079
Open Access Status Not yet assessed
Volume 55
Issue 2
Start page 110
End page 121
Total pages 12
Place of publication Hoboken
Publisher Wiley-Liss
Language eng
Subject C1
Abstract The strength of synaptic transmission is highly variable between different synapses. The present study examined some factors that may contribute to this variation in the strength of neurotransmission in sympathetic varicosities of the mouse vas deferens. Transmitter release was measured using a focal macropatch electrode placed over pairs of visualised varicosities. By regulating the calcium concentration of the solutions inside the recording electrode and in the bath independently of each other, transmitter release was restricted to one or two surface varicosities at each recording site. Using this technique, transmitter release probability was shown to be highly variable, even between adjacent varicosities on single axon branches. Very little variation was observed in the calcium influx following single impulse nerve stimulation between adjacent Oregon Green BAPTA-1 loaded varicosities. However, the staining intensities of three vesicular proteins, SV2, synaptophysin, and synaptotagmin 1, showed considerable variation between adjacent varicosities on single axon branches. This variation in staining intensity may be partly explained by variation in the density of synaptic vesicles. However, double staining experiments using two vesicular antigens showed some varicosities staining for one vesicular antigen, but not for the second, suggesting that the expression of these release machinery proteins is regulated locally within the varicosities. The results of the present study strengthen suggestions that synaptic strength is at least in part, regulated by variation in the expression of vesicular proteins. (C) 2004 Wiley-Liss, Inc.
Keyword Synaptic Strength
Synaptotagmin 1
Synaptic Vesicles
Mouse Vas-deferens
Sympathetic-nerve Varicosities
Smooth-muscle Cells
Quantal Secretion
Purinergic Transmission
Terminal Branches
Pyramidal Cells
Central Synapse
Q-Index Code C1
Grant ID 069768
Institutional Status UQ

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Created: Wed, 15 Aug 2007, 16:27:29 EST