Cutting edge: Species-specific TLR9-mediated recognition of CpG and non-CpG phosphorothioate-modified ohgonucleotides

Roberts, T. L., Sweet, M. J., Hume, D. A. and Stacey, K. J. (2005) Cutting edge: Species-specific TLR9-mediated recognition of CpG and non-CpG phosphorothioate-modified ohgonucleotides. Journal of Immunology, 174 2: 605-608.

Author Roberts, T. L.
Sweet, M. J.
Hume, D. A.
Stacey, K. J.
Title Cutting edge: Species-specific TLR9-mediated recognition of CpG and non-CpG phosphorothioate-modified ohgonucleotides
Journal name Journal of Immunology   Check publisher's open access policy
ISSN 0022-1767
Publication date 2005-01-01
Sub-type Article (original research)
Volume 174
Issue 2
Start page 605
End page 608
Total pages 4
Place of publication Bethesda
Publisher Amer Assoc Immunologists
Language eng
Subject C1
320202 Cellular Immunology
730102 Immune system and allergy
Abstract Different DNA motifs are required for optimal stimulation Of mouse and human immune cells by CpG oligode-oxynucleotides (ODN). These species differences presumably reflect sequence differences in TLR9, the CPG DNA receptor. In this study, we show that this sequence specificity is restricted to phosphorothioate (PS)-modified ODN and is not observed when a natural phosphodiester backbone is used. Thus, human and mouse cells have not evolved to recognize different CpG motifs in natural DNA. Nonoptimal PS-ODN (i.e., mouse CpG motif on human cells and vice versa) gave delayed and less sustained phosphorylation of p38 AWK than optimal motifs. When the CpG dinucleotide was inverted to GC In each ODN some residual activity of the PS-ODN was retained in a species-specific, TLR-9-dependent manner. Thus, TLR9 may he responsible for mediating many published CpG-independent responses to PS-ODN.
Keyword Immunology
Toll-like Receptor-9
Bacterial-dna
Immune Stimulation
Synthetic Oligodeoxynucleotides
In-vitro
B-cells
Activation
Motifs
Macrophages
Q-Index Code C1

 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 99 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 0 times in Scopus Article
Google Scholar Search Google Scholar
Created: Wed, 15 Aug 2007, 16:27:05 EST