The ABC transporter gene in the sirodesmin biosynthetic gene cluster of Leptosphaeria maculans is not essential for sirodesmin production but facilitates self-protection

Gardiner, Donald M., Jarvis, Renee S. and Howlett, Barbara J. (2005) The ABC transporter gene in the sirodesmin biosynthetic gene cluster of Leptosphaeria maculans is not essential for sirodesmin production but facilitates self-protection. Fungal Genetics And Biology, 42 3: 257-263. doi:10.1016/j.fgb.2004.12.001


Author Gardiner, Donald M.
Jarvis, Renee S.
Howlett, Barbara J.
Title The ABC transporter gene in the sirodesmin biosynthetic gene cluster of Leptosphaeria maculans is not essential for sirodesmin production but facilitates self-protection
Journal name Fungal Genetics And Biology   Check publisher's open access policy
ISSN 1087-1845
Publication date 2005-03-01
Sub-type Article (original research)
DOI 10.1016/j.fgb.2004.12.001
Open Access Status Not Open Access
Volume 42
Issue 3
Start page 257
End page 263
Total pages 7
Place of publication San Diego
Publisher Academic Press Inc Elsevier Science
Language eng
Subject C1
0607 Plant Biology
0604 Genetics
0605 Microbiology
Abstract Epipolythiodioxopiperazine toxins are secreted by a range of fungi, including Leptosphaeria maculans, which produces sirodesmin, and Aspergillus fumigatus, which produces gliotoxin. The L. maculans biosynthetic gene cluster for sirodesmin includes an ABC transporter gene, sirA. Disruption of this gene led to increased secretion of sirodesmin into the medium and an altered ratio of sirodesmin to its immediate precursor. The transcription pattern of a peptide synthetase that catalyses an early step in sirodesmin biosynthesis was elevated in the sirA mutant by 47% over a 7-day period. This was consistent with the finding that the transporter mutant had elevated sirodesmin levels. Despite increased production of sirodesmin, the sit-A mutant was more sensitive to both sirodesmin and gliotoxin. The putative gliotoxin transporter gene, gliA, (a major facilitator superfamily transporter) from A.fumigatus complemented the tolerance of the L. maculans sirA mutant to gliotoxin, but not to sirodesmin. The results indicate that SirA contributes to self-protection against sirodesmin in L. maculans and suggest a transporter other than SirA is primarily responsible for efflux of endogenously produced sirodesmin. (C) 2004 Elsevier Inc. All rights reserved.
Keyword Toxin
Aspergillus
Leptosphaeria
Gliotoxin
Sirodesmin
Etp
Transporter
Mfs
Abc
Secondary Metabolite
Genetics & Heredity
Mycology
Efflux Pump
Cercospora-kikuchii
Deficient Mutants
Fungal Toxin
Phoma-lingam
Pl
Phytotoxin
Expression
Epipolythiodioxopiperazines
Q-Index Code C1

 
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Created: Wed, 15 Aug 2007, 16:22:26 EST