Polarized trafficking of E-cadherin is regulated by Rac1 and Cdc42 in Madin-Darby canine kidney cells

Wang, B., Wylie, F. G., Teasdale, R. D. and Stow, J. L. (2005) Polarized trafficking of E-cadherin is regulated by Rac1 and Cdc42 in Madin-Darby canine kidney cells. American Journal of Physiology-cell Physiology, 288 6: C1411-C1419. doi:10.1152/ajpcell.00533.2004


Author Wang, B.
Wylie, F. G.
Teasdale, R. D.
Stow, J. L.
Title Polarized trafficking of E-cadherin is regulated by Rac1 and Cdc42 in Madin-Darby canine kidney cells
Journal name American Journal of Physiology-cell Physiology   Check publisher's open access policy
ISSN 0363-6143
Publication date 2005-01-01
Sub-type Article (original research)
DOI 10.1152/ajpcell.00533.2004
Volume 288
Issue 6
Start page C1411
End page C1419
Total pages 9
Place of publication Bethesda
Publisher Amer Physiological Soc
Language eng
Subject C1
270103 Protein Targeting and Signal Transduction
780106 Political science and public policy
Abstract E-cadherin is a major cell-cell adhesion protein of epithelia that is trafficked to the basolateral cell surface in a polarized fashion. The exact post-Golgi route and regulation of E-cadherin transport have not been fully described. The Rho GTPases Cdc42 and Rac1 have been implicated in many cell functions, including the exocytic trafficking of other proteins in polarized epithelial cells. These Rho family proteins are also associated with the cadherin-catenin complexes at the cell surface. We have used functional mutants of Rac1 and Cdc42 and inactivating toxins to demonstrate specific roles for both Cdc42 and Rac1 in the post-Golgi transport of E-cadherin. Dominant-negative mutants of Cdc42 and Rac1 accumulate E-cadherin at a distinct post-Golgi step. This accumulation occurs before p120(ctn) interacts with E-cadherin, because p120(ctn) localization was not affected by the Cdc42 or Rac1 mutants. Moreover, the GTPase mutants had no effect on the trafficking of a targeting mutant of E-cadherin, consistent with the selective involvement of Cdc42 and Rac1 in basolateral trafficking. These results provide a new example of Rho GTPase regulation of basolateral trafficking and demonstrate novel roles for Cdc42 and Rac1 in the post-Golgi transport of E-cadherin.
Keyword Rho Family Gtpases
Catenin
Polarity
Sorting
Actin
Cell Biology
Physiology
Basolateral Plasma-membrane
Trans-golgi Network
Basal-lateral Membrane
Epithelial-cells
Catenin Complexes
Rho-proteins
Mdck Cells
Gtpases
Roles
Q-Index Code C1

 
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Created: Wed, 15 Aug 2007, 16:12:26 EST