The phasevarion: A genetic system controlling coordinated, random switching of expression of multiple genes

Srikhanta, Y. N., Maguire, T. L., Stacey, K. J., Grimmond, S. M. and Jennings, M. P. (2005) The phasevarion: A genetic system controlling coordinated, random switching of expression of multiple genes. Proceedings of The National Academy of Sciences of The United States of America, 102 15: 5547-5551. doi:10.1073/pnas.0501169102


Author Srikhanta, Y. N.
Maguire, T. L.
Stacey, K. J.
Grimmond, S. M.
Jennings, M. P.
Title The phasevarion: A genetic system controlling coordinated, random switching of expression of multiple genes
Journal name Proceedings of The National Academy of Sciences of The United States of America   Check publisher's open access policy
ISSN 0027-8424
Publication date 2005-01-01
Sub-type Article (original research)
DOI 10.1073/pnas.0501169102
Volume 102
Issue 15
Start page 5547
End page 5551
Total pages 5
Place of publication Washington, D.C.
Publisher National Academy of Sciences of the U.S.
Language eng
Subject C1
270306 Microbial Genetics
730101 Infectious diseases
Abstract Several host-adapted bacterial pathogens contain methyltransferases associated with type III restriction-modification (R-M) systems that are subject to reversible, high-frequency on/off switching of expression (phase variation). To investigate the role of phase-variable expression of R-M systems, we made a mutant strain lacking the methyltransferase (mod) associated with a type III R-M system of Haemophilus influenzae and analyzed its phenotype. By microarray analysis, we identified a number of genes that were either up- or down-regulated in the mod mutant strain. This system reports the coordinated random switching of a set of genes in a bacterial pathogen and may represent a widely used mechanism.
Keyword DNA Methyltransferase
Haemophilus Influenzae
Phase Variation
Microbiology
Restriction-modification System
Outer-membrane Protein
Dna Adenine Methylase
Haemophilus-influenzae
Neisseria-meningitidis
Variable Expression
Lipopolysaccharide
Repeat
Hemoglobin
Multidisciplinary Sciences
Q-Index Code C1

 
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Created: Wed, 15 Aug 2007, 16:12:20 EST