C/EBP delta and C/EBP gamma bind the CCAAT-box in the human beta-globin promoter and modulate the activity of the CACC-box binding protein, EKLF

Gordon, Christopher T., Fox, Venessa J., Najdovska, Suzana and Perkins, Andrew C. (2005) C/EBP delta and C/EBP gamma bind the CCAAT-box in the human beta-globin promoter and modulate the activity of the CACC-box binding protein, EKLF. Biochimica et biophysica acta (BBA). Gene structure and expression, 1729 1: 74-80. doi:10.1016/j.bbaexp.2005.03.004


Author Gordon, Christopher T.
Fox, Venessa J.
Najdovska, Suzana
Perkins, Andrew C.
Title C/EBP delta and C/EBP gamma bind the CCAAT-box in the human beta-globin promoter and modulate the activity of the CACC-box binding protein, EKLF
Journal name Biochimica et biophysica acta (BBA). Gene structure and expression   Check publisher's open access policy
ISSN 0167-4781
Publication date 2005-05-25
Year available 2005
Sub-type Article (original research)
DOI 10.1016/j.bbaexp.2005.03.004
Open Access Status Not yet assessed
Volume 1729
Issue 1
Start page 74
End page 80
Total pages 7
Place of publication Amsterdam
Publisher Elsevier Biomedical Press
Language eng
Subject C1
270201 Gene Expression
730103 Blood disorders
Abstract Developmental- and tissue-specific expression of globin genes is mediated by a few key elements within the proximal promoter of each gene. DNA-binding assays previously identified NF-Y, GATA-1, C/EBP beta and C/EBP gamma as candidate regulators of beta-globin transcription via the CCAAT-box, a promoter element situated between CACC- and TATA-boxes. We have identified C/EBP delta as an additional beta-globin CCAAT-box binding protein. In reporter assays, we show that C/EBP delta can co-operate with EKLF, a CACC-box binding protein, to activate the beta-globin promoter, whereas C/EBP gamma inhibits the transcriptional activity of EKLF in this assay. (c) 2005 Elsevier B.V. All rights reserved.
Keyword Ccaat-box
C/ebp Delta
C/ebp Gamma
Eklf
Beta-globin Promoter
Luciferase Assay
Biochemistry & Molecular Biology
Biophysics
Murine Erythroleukemia-cells
Transcription Factor Gata-1
Locus-control Region
Major Basic-protein
Gene-expression
Mice Lacking
Activator Protein
Family Proteins
Kruppel Family
K562 Cells
Q-Index Code C1
Institutional Status UQ
Additional Notes DOI: 10.1016/j.bbaexp.2005.03.004

 
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Created: Wed, 15 Aug 2007, 16:09:30 EST