Method for the generation and cultivation of functional three-dimensional mammary constructs without exogenous extracellular matrix

Timmins, N. E., Harding, F. J., Smart, C. E., Brown, M. A. and Nielsen, L. K. (2005) Method for the generation and cultivation of functional three-dimensional mammary constructs without exogenous extracellular matrix. Cell And Tissue Research, 320 1: 207-210. doi:10.1007/s00441-004-1064-6


Author Timmins, N. E.
Harding, F. J.
Smart, C. E.
Brown, M. A.
Nielsen, L. K.
Title Method for the generation and cultivation of functional three-dimensional mammary constructs without exogenous extracellular matrix
Journal name Cell And Tissue Research   Check publisher's open access policy
ISSN 0302-766X
Publication date 2005-01-01
Year available 2005
Sub-type Article (original research)
DOI 10.1007/s00441-004-1064-6
Open Access Status Not yet assessed
Volume 320
Issue 1
Start page 207
End page 210
Total pages 4
Editor K. Unsicker
Place of publication New York
Publisher Springer
Language eng
Subject C1
270303 Virology
770804 Control of pests and exotic species
Abstract During puberty, pregnancy, lactation and postlactation, breast tissue undergoes extensive remodelling and the disruption of these events can lead to cancer. In vitro studies of mammary tissue and its malignant transformation regularly employ mammary epithelial cells cultivated on matrigel or floating collagen rafts. In these cultures, mammary epithelial cells assemble into three-dimensional structures resembling in vivo acini. We present a novel technique for generating functional mammary constructs without the use of matrix substitutes.
Keyword Cell Biology
Mammary Epithelial Cell
Breast Tissue
Mammary Acini
Breast Cancer
Three-dimensional Culture
Mouse (c57b1/6xcba/f1)
Gland Development
Gene-expression
Differentiation
Microenvironment
Cells
Ecm
Q-Index Code C1
Institutional Status UQ

 
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Created: Wed, 15 Aug 2007, 16:09:23 EST