The rat Na+-sulfate cotransporter rNaS2: functional characterization, tissue distribution, and gene (slc13a4) structure

Dawson, P. A., Pirlo, K. J., Steane, S. E., Nguyen, K. A., Kunzelmann, K., Chien, Y. J. and Markovich, D. (2005) The rat Na+-sulfate cotransporter rNaS2: functional characterization, tissue distribution, and gene (slc13a4) structure. Pflugers Archiv-european Journal of Physiology, 450 4: 262-268. doi:10.1007/s00424-005-1414-6


Author Dawson, P. A.
Pirlo, K. J.
Steane, S. E.
Nguyen, K. A.
Kunzelmann, K.
Chien, Y. J.
Markovich, D.
Title The rat Na+-sulfate cotransporter rNaS2: functional characterization, tissue distribution, and gene (slc13a4) structure
Journal name Pflugers Archiv-european Journal of Physiology   Check publisher's open access policy
ISSN 0031-6768
Publication date 2005-01-01
Sub-type Article (original research)
DOI 10.1007/s00424-005-1414-6
Open Access Status Not Open Access
Volume 450
Issue 4
Start page 262
End page 268
Total pages 7
Editor B. Nilius
Place of publication New York
Publisher Springer
Language eng
Subject C1
270201 Gene Expression
780105 Biological sciences
Abstract Inorganic sulfate is essential for numerous functions in mammalian physiology. In the present study, we characterized the functional properties of the rat Na+-sulfate cotransporter NaS2 (rNaS2), determined its tissue distribution, and identified its gene (slc13a4) structure. Expression of rNaS2 protein in Xenopus oocytes led to a Na+-dependent transport of sulfate that was inhibited by phosphate, thiosulfate, tungstate, selenate, oxalate, and molybdate, but not by citrate, succinate, or DIDS. Transport kinetics of rNaS2 determined a K-M for sulfate of 1.26 mM. Na+ kinetics determined a Hill coefficient of n=3.0 +/- 0.7, suggesting a Na+:SO42- stoichiometry of 3:1. rNaS2 mRNA was highly expressed in placenta, with lower levels found in the brain and liver. slc13a4 maps to rat chromosome 4 and contains 17 exons, spanning over 46 kb in length. This gene produces two alternatively spliced transcripts, of which the transcript lacking exon 2 is the most abundant form. Its 5' flanking region contains CAAT- and GC-box motifs and a number of putative transcription factor binding sites, including GATA-1, SP1, and AP-2 consensus sequences. This is the first study to characterize rNaS2 transport kinetics, define its tissue distribution, and resolve its gene (slc13a4) structure and 5' flanking region.
Keyword Sulfate
Sodium Sulfate Cotransport
Slc13a4
Tissue Distribution
Gene Structure
Physiology
Transcriptional Regulation
Expression Cloning
Transporter
Nasi-1
Mouse
Proteoglycans
Organization
Slc26a1
Protein
Brain
Q-Index Code C1
Institutional Status UQ

 
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Created: Wed, 15 Aug 2007, 16:01:49 EST