Cosuppression of eukaryotic release factor 1-1 in Arabidopsis affects cell elongation and radial cell division

Petsch, K. A., Botella, J. R. and Mylne, J. (2005) Cosuppression of eukaryotic release factor 1-1 in Arabidopsis affects cell elongation and radial cell division. Plant Physiology, 139 1: 115-126. doi:10.1104/pp.105.062695


Author Petsch, K. A.
Botella, J. R.
Mylne, J.
Title Cosuppression of eukaryotic release factor 1-1 in Arabidopsis affects cell elongation and radial cell division
Journal name Plant Physiology   Check publisher's open access policy
ISSN 0032-0889
Publication date 2005-01-01
Year available 2005
Sub-type Article (original research)
DOI 10.1104/pp.105.062695
Open Access Status PMC
Volume 139
Issue 1
Start page 115
End page 126
Total pages 12
Editor Natasha V Raikhel
Melissa Junior
Place of publication Rockville
Publisher Amer Soc Plant Biologists
Language eng
Subject C1
270402 Plant Physiology
620200 Horticultural Crops
Abstract The role of the eukaryotic release factor 1 (eRF1) in translation termination has previously been established in yeast; however, only limited characterization has been performed on any plant homologs. Here, we demonstrate that cosuppression of eRF1-1 in Arabidopsis (Arabidopsis thaliana) has a profound effect on plant morphology, resulting in what we term the broomhead phenotype. These plants primarily exhibit a reduction in internode elongation causing the formation of a broomhead-like cluster of malformed siliques at the top of the inflorescence stem. Histological analysis of broomhead stems revealed that cells are reduced in height and display ectopic lignification of the phloem cap cells, some phloem sieve cells, and regions of the fascicular cambium, as well as enhanced lignification of the interfascicular fibers. We also show that cell division in the fascicular cambial regions is altered, with the majority of vascular bundles containing cambial cells that are disorganized and possess enlarged nuclei. This is the first attempt at functional characterization of a release factor in vivo in plants and demonstrates the importance of eRF1-1 function in Arabidopsis.
Keyword Plant Sciences
Translation Termination Factors
Yeast Saccharomyces-cerevisiae
Transgenic Tobacco Plants
Peptide-chain Termination
Factor Erf1
Rna Degradation
Co-suppression
Nitrate Reductase
Sup45 Genes
Expression
Q-Index Code C1
Institutional Status UQ

 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 18 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 18 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Wed, 15 Aug 2007, 15:54:25 EST