Gene codon composition determines differentiation-dependent expression of a viral capsid gene in keratinocytes in vitro and in vivo

Zhao, Kong-Nan, Gu, WenYi, Fang, Ning Xia, Saunders, Nicholas A. and Frazer, Ian H. (2005) Gene codon composition determines differentiation-dependent expression of a viral capsid gene in keratinocytes in vitro and in vivo. Molecular And Cellular Biology, 25 19: 8643-8655. doi:10.1128/MCB.25.19.8643-8655.2005

Attached Files (Some files may be inaccessible until you login with your UQ eSpace credentials)
Name Description MIMEType Size Downloads
UQ75356_OA.pdf Full text (open access) application/pdf 1.26MB 0

Author Zhao, Kong-Nan
Gu, WenYi
Fang, Ning Xia
Saunders, Nicholas A.
Frazer, Ian H.
Title Gene codon composition determines differentiation-dependent expression of a viral capsid gene in keratinocytes in vitro and in vivo
Journal name Molecular And Cellular Biology   Check publisher's open access policy
ISSN 0270-7306
1098-5549
Publication date 2005-10-01
Year available 2005
Sub-type Article (original research)
DOI 10.1128/MCB.25.19.8643-8655.2005
Open Access Status File (Publisher version)
Volume 25
Issue 19
Start page 8643
End page 8655
Total pages 13
Place of publication Washington, DC, United States
Publisher American Society for Microbiology
Language eng
Abstract By establishing mouse primary keratinocytes (KCs) in culture, we were able, for the first time, to express papillomavirus major capsid (L1) proteins by transient transfection of authentic or codon-modified L1 gene expression plasmids. We demonstrate in vitro and in vivo that gene codon composition is in part responsible for differentiation-dependent expression of L1 protein in KCs. L1 mRNA was present in similar amounts in differentiated and undifferentiated KCs transfected with authentic or codon-modified L1 genes and had a similar half-life, demonstrating that L1 protein production is posttranscriptionally regulated. We demonstrate further that KCs substantially change their tRNA profiles upon differentiation. Aminoacyl-tRNAs from differentiated KCs but not undifferentiated KCs enhanced the translation of authentic L1 mRNA, suggesting that differentiation-associated change to tRNA profiles enhances L1 expression in differentiated KCs. Thus, our data reveal a novel mechanism for regulation of gene expression utilized by a virus to direct viral capsid protein expression to the site of virion assembly in mature KCs. Analysis of two structural proteins of KCs, involucrin and keratin 14, suggests that translation of their mRNAs is also regulated, in association with KC differentiation in vitro, by a similar mechanism
Keyword Human-papillomavirus type-16
Rna instability elements
3 untranslated region
Coding region
Saccharomyces-cerevisiae
Messenger-rnas
Protein-synthesis
Epithelial-cells
Mammalian-cells
Down-regulation
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 40 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 42 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Wed, 15 Aug 2007, 15:48:11 EST