Sunscreen penetration of human skin and related keratinocyte toxicity after topical application

Hayden, C. G. J., Cross, S. E., Anderson, C., Saunders, N. A. and Roberts, M. S. (2005) Sunscreen penetration of human skin and related keratinocyte toxicity after topical application. Skin Pharmacology And Physiology, 18 4: 170-174. doi:10.1159/000085861

Author Hayden, C. G. J.
Cross, S. E.
Anderson, C.
Saunders, N. A.
Roberts, M. S.
Title Sunscreen penetration of human skin and related keratinocyte toxicity after topical application
Journal name Skin Pharmacology And Physiology   Check publisher's open access policy
ISSN 1660-5527
Publication date 2005-01-01
Sub-type Article (original research)
DOI 10.1159/000085861
Volume 18
Issue 4
Start page 170
End page 174
Total pages 5
Editor J. Lademann
Place of publication Switzerland
Publisher S. Karger AG
Language eng
Subject C1
320599 Pharmacology not elsewhere classified
730117 Skin and related disorders
Abstract Sunscreen skin penetration and safety assessment should be considered together in order to ensure that in vitro cytotoxicity studies examine relevant doses of these organic chemical UV filters to which viable epidermal cells are realistically exposed. In this study, we sought to determine whether sufficient topically applied sunscreens penetrated into human viable epidermis to put the local keratinocyte cell populations at risk of toxicity. The penetration and retention of five commonly used sunscreen agents ( avobenzone, octinoxate, octocrylene, oxybenzone and padimate O) in human skin was evaluated after application in mineral oil to isolated human epidermal membranes. Sunscreen concentration - human keratinocyte culture response curves were then defined using changes in cell morphology and proliferation ( DNA synthesis using radiolabelled thymidine uptake studies) as evidence of sunscreens causing toxicity. Following 24 h of human epidermal exposure to sunscreens, detectable amounts of all sunscreens were present in the stratum corneum and viable epidermis, with epidermal penetration most evident with oxybenzone. The concentrations of each sunscreen found in human viable epidermis after topical application, adjusting for skin partitioning and binding effects, were at least 5-fold lower, based on levels detected in viable epidermal cells, than those appearing to cause toxicity in cultured human keratinocytes. It is concluded that the human viable epidermal levels of sunscreens are too low to cause any significant toxicity to the underlying human keratinocytes. Copyright (C) 2005 S. Karger AG, Basel.
Keyword Pharmacology & Pharmacy
Uv Filters
Human Viable Epidermis
Q-Index Code C1

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Created: Wed, 15 Aug 2007, 15:40:51 EST