Inhibition of interferon signaling by the New York 99 strain and Kunjin subtype of West Nile virus involves blockage of STAT1 and STAT2 activation by nonstructural proteins

Liu, Wen Jun, Wang, Xiang Ju, Mokhonov, Vladislav V., Shi, Pei-Yong, Randall, Richard and Khromykh, Alexander A. (2005) Inhibition of interferon signaling by the New York 99 strain and Kunjin subtype of West Nile virus involves blockage of STAT1 and STAT2 activation by nonstructural proteins. Journal of Virology, 79 3: 1934-1942. doi:10.1128/JVI.79.3.1934-1942.2005


Author Liu, Wen Jun
Wang, Xiang Ju
Mokhonov, Vladislav V.
Shi, Pei-Yong
Randall, Richard
Khromykh, Alexander A.
Title Inhibition of interferon signaling by the New York 99 strain and Kunjin subtype of West Nile virus involves blockage of STAT1 and STAT2 activation by nonstructural proteins
Journal name Journal of Virology   Check publisher's open access policy
ISSN 0022-538X
1098-5514
Publication date 2005-02-01
Year available 2005
Sub-type Article (original research)
DOI 10.1128/JVI.79.3.1934-1942.2005
Open Access Status DOI
Volume 79
Issue 3
Start page 1934
End page 1942
Total pages 9
Place of publication Washington, DC, United States
Publisher American Society for Microbiology
Collection year 2005
Language eng
Subject C1
270303 Virology
730101 Infectious diseases
Abstract The interferon (IFN) response is the first line of defense against viral infections, and the majority of viruses have developed different strategies to counteract IFN responses in order to ensure their survival in an infected host. In this study, the abilities to inhibit IFN signaling of two closely related West Nile viruses, the New York 99 strain (NY99) and Kunjin virus (KUN), strain MRM61C, were analyzed using reporter plasmid assays, as well as immunofluorescence and Western blot analyses. We have demonstrated that infections with both NY99 and KUN, as well as transient or stable transfections with their replicon RNAs, inhibited the signaling of both alpha/beta IFN (IFN-alpha/beta) and gamma IFN (IFN-gamma) by blocking the phosphorylation of STAT1 and its translocation to the nucleus. In addition, the phosphorylation of STAT2 and its translocation to the nucleus were also blocked by KUN, NY99, and their replicons in response to treatment with IFN-alpha. IFN-alpha signaling and STAT2 translocation to the nucleus was inhibited when the KUN nonstructural proteins NS2A, NS2B, NS3, NS4A, and NS4B, but not NS1 and NS5, were expressed individually from the pcDNA3 vector. The results clearly demonstrate that both NY99 and KUN inhibit IFN signaling by preventing STAT1 and STAT2 phosphorylation and identify nonstructural proteins. responsible for this inhibition.
Keyword Kunjin
West Nile Virus
Interferon Signaling
New York 99 Strain
Nonstructural Proteins
Virology
Regulatory Factor-3
Subgenomic Replicons
Dengue Virus
V-protein
Beta
Replication
Expression
Infection
Responses
Pathway
Q-Index Code C1
Institutional Status UQ

 
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Created: Wed, 15 Aug 2007, 15:37:20 EST