Hybrid 'Sinta' papaya exhibits unique ACC synthase 1 cDNA isoforms

Hidalgo, M. S. P., Tecson-Mendoza, E. M., Laurena, A. C. and Botella, J. R. (2005) Hybrid 'Sinta' papaya exhibits unique ACC synthase 1 cDNA isoforms. Journal of Biochemistry And Molecular Biology, 38 3: 320-327.

Author Hidalgo, M. S. P.
Tecson-Mendoza, E. M.
Laurena, A. C.
Botella, J. R.
Title Hybrid 'Sinta' papaya exhibits unique ACC synthase 1 cDNA isoforms
Journal name Journal of Biochemistry And Molecular Biology   Check publisher's open access policy
ISSN 1225-8687
Publication date 2005-01-01
Year available 2005
Sub-type Article (original research)
Open Access Status Not yet assessed
Volume 38
Issue 3
Start page 320
End page 327
Total pages 8
Editor Changwon Kang
Place of publication Singapore
Publisher Springer Singapore Pte Ltd
Language eng
Subject C1
270400 Botany
620100 Field Crops
Abstract Five ripening-related ACC synthase cDNA isoforms were cloned from 80% ripe papaya cv. 'Sinta' by reverse transcription-PCR using gene-specific primers. Clone 2 had the longest transcript and contained all common exons and three alternative exons. Clones 3 and 4 contained common exons and one alternative exon each, while clone 1, the most common transcript, contained only the common exons. Clone 5 could be due to cloning artifacts and might not be a unique cDNA fragment. Thus, there are only four isoforms of ACC synthase mRNA. Southern blot analysis indicates that all five clones came from only one gene existing as a single copy in the 'Sinta' papaya genome. Multiple sequence alignment indicates that the four isoforms arise from a single gene, possibly through alternative splicing mechanisms. All the putative alternative exons were present at the 5'-end of the gene comprising the N-terminal region of the protein. 'Sinta' ACC synthase cDNAs were of the capacs 1 type and are most closely related to a 1.4 kb capacs 1-type DNA (AJ277160) from Eksotika papaya. No capacs 2-type cDNAs were cloned from 'Sinta' by RT-PCR. This is the first report of possible alternative splicing mechanism in ripening-related ACC synthase genes in hybrid papaya, possibly to modulate or fine-tune gene expression relevant to fruit ripening.
Keyword Biochemistry & Molecular Biology
Acc Synthase
Capacs 1
Carica Papaya
Papaya
Induced 1-aminocyclopropane-1-carboxylate Synthase
Differential Expression
Escherichia-coli
Tomato Plants
Acid Synthase
2 Genes
Fruit
Transcripts
Senescence
Ethylene
Q-Index Code C1
Institutional Status UQ

 
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Created: Wed, 15 Aug 2007, 15:26:16 EST