Organization of the AAA(+) adaptor protein PspA is an oligomeric ring

Hankamer, B. D., Elderkin, S. L., Buck, M. and Nield, J. (2004) Organization of the AAA(+) adaptor protein PspA is an oligomeric ring. Journal of Biological Chemistry, 279 10: 8862-8866. doi:10.1074/jbc.M307889200

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Author Hankamer, B. D.
Elderkin, S. L.
Buck, M.
Nield, J.
Title Organization of the AAA(+) adaptor protein PspA is an oligomeric ring
Formatted title
Organization of the AAA+ adaptor protein PspA is an oligomeric ring
Journal name Journal of Biological Chemistry   Check publisher's open access policy
ISSN 0021-9258
Publication date 2004-03-05
Sub-type Article (original research)
DOI 10.1074/jbc.M307889200
Open Access Status File (Publisher version)
Volume 279
Issue 10
Start page 8862
End page 8866
Total pages 5
Place of publication Bethesda, MD, U.S.A.
Publisher American Society for Biochemistry and Molecular Biology, Inc
Language eng
Subject C1
270104 Membrane Biology
780105 Biological sciences
0601 Biochemistry and Cell Biology
0904 Chemical Engineering
Formatted abstract
The 25.3 kDa "adaptor" protein, PspA (phage shock protein A), is found in the cytoplasm and in association with the inner membrane of certain bacteria. PspA plays critical roles in negatively regulating the phage shock response and maintaining membrane integrity, especially during the export of proteins such as virulence factors. Homologues of PspA function exist for thylakoid biogenesis. Here we report the first three-dimensional reconstruction of a PspA assembly from Escherichia coli, visualized by electron microscopy and single particle analysis to a resolution of 30 Å. The assembly forms a 9-fold rotationally symmetric ring with an outer diameter of 200 Å, an inner diameter of 95 Å, and a height of ∼85 Å. The molecular mass of the complex was calculated to be 1023 kDa by size exclusion chromatography, suggesting that each of the nine domains is likely to be composed of four PspA subunits. The functional implications of this PspA structure are discussed in terms of its interaction with the protein export machinery of the bacterial cell and its AAA+ protein partner, PspF.
Keyword Biochemistry & molecular biology
Cryoelectron microscopy
Q-Index Code C1

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Created: Wed, 15 Aug 2007, 14:50:31 EST