Identification of a Golgi-localised GRIP domain protein from Arabidopsis thaliana

Gilson, Paul R., Vergara, Claudia E., Kjer-Nielsen, Lars, Teasdale, Rohan D., Bacic, Antony and Gleeson, Paul A. (2004) Identification of a Golgi-localised GRIP domain protein from Arabidopsis thaliana. Planta, 219 6: 1050-1056. doi:10.1007/s00425-004-1311-9

Author Gilson, Paul R.
Vergara, Claudia E.
Kjer-Nielsen, Lars
Teasdale, Rohan D.
Bacic, Antony
Gleeson, Paul A.
Title Identification of a Golgi-localised GRIP domain protein from Arabidopsis thaliana
Journal name Planta   Check publisher's open access policy
ISSN 0032-0935
Publication date 2004-01-01
Year available 2004
Sub-type Article (original research)
DOI 10.1007/s00425-004-1311-9
Open Access Status Not yet assessed
Volume 219
Issue 6
Start page 1050
End page 1056
Total pages 7
Place of publication Berlin
Publisher Springer-Verlag
Language eng
Subject C1
270200 Genetics
780105 Biological sciences
Abstract A family of Golgi-localised molecules was recently described in animals and fungi possessing extensive coiled regions and a short (similar to40 residues) conserved C-terminal domain, called the GRIP domain, which is responsible for their location to this organelle. Using the model plant Arabidopsis thaliana, we identified a gene (AtGRIP) encoding a putative GRIP protein. We demonstrated that the C-terminal domain from AtGRIP functions as a Golgi-targeting sequence in plant cells. Localisation studies in living cells expressing the AtGRIP fused to a DsRed2 fluorescent probe, showed extensive co-location with the Golgi marker alpha-mannosidase I in transformed tobacco protoplasts. GRIP-like sequences were also found in genomic databases of rice, maize, wheat and alfalfa, suggesting that this domain may be a useful Golgi marker for immunolocalisation studies. Despite low sequence identity amongst GRIP domains, the plant GRIP sequence was able to target to the Golgi of mammalian cells. Taken together, these data indicate that GRIP domain proteins might be implicated in a targeting mechanism that is conserved amongst eukaryotes.
Keyword Plant Sciences
Golgi Apparatus
Grip Domain
Coiled-coil Proteins
Molecular Characterization
Q-Index Code C1
Institutional Status UQ

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Created: Wed, 15 Aug 2007, 14:44:46 EST