Membrane insertion of anthrax protective antigen and cytoplasmic delivery of lethal factor occur at different stages of the endocytic pathway

Abrami, L., Lindsay, M., Parton, R. G., Leppla, S. H. and van der Goot, F. G. (2004) Membrane insertion of anthrax protective antigen and cytoplasmic delivery of lethal factor occur at different stages of the endocytic pathway. Journal of Cell Biology, 166 5: 645-651. doi:10.1083/jcb.200312072

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Author Abrami, L.
Lindsay, M.
Parton, R. G.
Leppla, S. H.
van der Goot, F. G.
Title Membrane insertion of anthrax protective antigen and cytoplasmic delivery of lethal factor occur at different stages of the endocytic pathway
Journal name Journal of Cell Biology   Check publisher's open access policy
ISSN 0021-9525
1540-8140
Publication date 2004-01-01
Year available 2004
Sub-type Article (original research)
DOI 10.1083/jcb.200312072
Open Access Status File (Publisher version)
Volume 166
Issue 5
Start page 645
End page 651
Total pages 7
Editor Tom Misteli
Place of publication New York, NY, United States
Publisher Rockefeller University Press
Language eng
Abstract The protective antigen (PA) of anthrax toxin binds to a cell surface receptor, undergoes heptamerization, and binds the enzymatic subunits, the lethal factor (LF) and the edema factor (EF). The resulting complex is then endocytosed. Via mechanisms that depend on the vacuolar ATPase and require membrane insertion of PA, LF and EF are ultimately delivered to the cytoplasm where their targets reside. Here, we show that membrane insertion of PA already occurs in early endosomes, possibly only in the multivesicular regions, but that subsequent delivery of LF to the cytoplasm occurs preferentially later in the endocytic pathway and relies on the dynamics of internal vesicles of multivesicular late endosomes.
Keyword Cell Biology
Cell Biology
CELL BIOLOGY
Q-Index Code C1
Grant ID AI053270-01
Institutional Status UQ

 
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Created: Wed, 15 Aug 2007, 14:35:15 EST