Expression of the tudor-related gene Tdrd5 during development of the male germline in mice

Smith, J. M., Bowles, J., Wilson, M., Teasdale, R. D. and Koopman, P. (2004) Expression of the tudor-related gene Tdrd5 during development of the male germline in mice. Gene Expression Patterns, 4 6: 701-705. doi:10.1016/j.modgep.2004.04.002


Author Smith, J. M.
Bowles, J.
Wilson, M.
Teasdale, R. D.
Koopman, P.
Title Expression of the tudor-related gene Tdrd5 during development of the male germline in mice
Journal name Gene Expression Patterns   Check publisher's open access policy
ISSN 1567-133X
ISBN 1567-133x
Publication date 2004-01-01
Sub-type Article (original research)
DOI 10.1016/j.modgep.2004.04.002
Volume 4
Issue 6
Start page 701
End page 705
Total pages 5
Place of publication Netherlands
Publisher Elsevier BV
Language eng
Subject C1
270205 Genetic Development (incl. Sex Determination)
780105 Biological sciences
Abstract Homologues of Drosophila germ cell determinant genes such as vasa, nanos and tudor have recently been implicated in development of the male germline in mice. In the present study, the mouse gene encoding Tudor domain containing protein 5 (TDRD5) was isolated from a 12.5-13.5 days post coitum (dpc) male-enriched subtracted cDNA library. Whole-mount in situ hybridization analysis of Tdrd5 expression in the mouse embryonic gonad indicated that this gene is upregulated in the developing testis from 12.5 dpc, with expression levels remaining higher in testis than ovary throughout embryogenesis. Expression of Tdrd5 was absent in testes isolated from W-e/W-e embryos, which lack germ cells. In situ hybridization (ISH) on cryosectioned 13.5 dpc testes suggests that expression of Tdrd5, like that of Oct4, is restricted to germ cells. Northern hybridization analysis of expression in adult tissues indicated that Tdrd5 is expressed in the testis only, implying that expression of this gene is restricted to the male germline throughout development to adulthood. (C) 2004 Elsevier B.V. All rights reserved.
Keyword Developmental Biology
Genetics & Heredity
Germ Cells
Tudor Domain Containing Protein 5
Tudor Domain
Testis
Gonad Development
Cells
Complex
Hybridization
Suggests
Female
Q-Index Code C1

 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 18 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 19 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Wed, 15 Aug 2007, 14:34:49 EST