Determination of the extraction kinetics for the quantification of polyhydroxyalkanoate monomers in mixed microbial systems

Lanham, Ana B., Ricardo, Ana Rita, Albuquerque, Maria G. E., Pardelha, Filipa, Carvalheira, Monica, Coma, Marta, Fradinho, Joana, Carvalho, Gilda, Oehmen, Adrian and Reis, Maria A. M. (2013) Determination of the extraction kinetics for the quantification of polyhydroxyalkanoate monomers in mixed microbial systems. Process Biochemistry, 48 11: 1626-1634. doi:10.1016/j.procbio.2013.07.023


Author Lanham, Ana B.
Ricardo, Ana Rita
Albuquerque, Maria G. E.
Pardelha, Filipa
Carvalheira, Monica
Coma, Marta
Fradinho, Joana
Carvalho, Gilda
Oehmen, Adrian
Reis, Maria A. M.
Title Determination of the extraction kinetics for the quantification of polyhydroxyalkanoate monomers in mixed microbial systems
Journal name Process Biochemistry   Check publisher's open access policy
ISSN 1359-5113
1873-3298
Publication date 2013-11-01
Sub-type Article (original research)
DOI 10.1016/j.procbio.2013.07.023
Open Access Status Not yet assessed
Volume 48
Issue 11
Start page 1626
End page 1634
Total pages 9
Place of publication London, United Kingdom
Publisher Elsevier
Language eng
Abstract For the first time, a systematic approach was conducted to determine the key factors influencing the kinetics of hydroxyalkanote (HA) extraction in biological systems. Six mixed microbial systems where polyhydroxyalkanoate (PHA) is produced were evaluated. Experiments were carried out for full-scale and lab-scale activated sludge systems using different configurations (containing floccular or granular sludge), as well as specific PHA accumulating cultures that contain high or low intracellular PHA fractions. The overall reaction was limited by the kinetics of the PHA hydrolysis in floccular cultures, whereas in granular cultures, it was limited by the cell lysis step. The monomeric composition of the polymer also had an impact on the HA extraction rate: higher acid concentration and a longer digestion time should be employed when cells accumulate monomers with more substituents, such as hydroxy-2-methylbutyrate (H2MB) and hydroxy-2-methylvalerate (H2MV). This study optimised the method for HA extraction, which impacts the assessment of the quantity and quality of PHA biopolymers.
Keyword Extraction and recovery
Polyhydroxy-2- methylbutyrate (PH2MB)
Polyhydroxy-2-methylvalerate (PH2MV)
Polyhydroxybutyrate (PHB)
Polyhydroxyvalerate (PHV)
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Chemical Engineering Publications
 
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Created: Wed, 09 May 2018, 16:35:00 EST by Ms Kirsty Fraser on behalf of School of Chemical Engineering