Sperm chromatin dispersion test (SCDt) for the assessment of sperm DNA fragmentation in black tiger prawn, Penaeus monodon

Feng, Tianyi, Gosálvez, Jamie, Lopez-Fernandez, Carmen, Arroyo, Francisca, Paterson, Brian and Johnston, Stephen (2018) Sperm chromatin dispersion test (SCDt) for the assessment of sperm DNA fragmentation in black tiger prawn, Penaeus monodon. Aquaculture, 491 281-288. doi:10.1016/j.aquaculture.2018.03.041


Author Feng, Tianyi
Gosálvez, Jamie
Lopez-Fernandez, Carmen
Arroyo, Francisca
Paterson, Brian
Johnston, Stephen
Title Sperm chromatin dispersion test (SCDt) for the assessment of sperm DNA fragmentation in black tiger prawn, Penaeus monodon
Journal name Aquaculture   Check publisher's open access policy
ISSN 0044-8486
1873-5622
Publication date 2018-04-01
Sub-type Article (original research)
DOI 10.1016/j.aquaculture.2018.03.041
Open Access Status Not yet assessed
Volume 491
Start page 281
End page 288
Total pages 8
Place of publication Amsterdam, NX Netherlands
Publisher Elsevier
Language eng
Subject 1104 Aquatic Science
Abstract The decapod sperm cell possesses no flagellum so that sperm evaluation has been primarily limited to the integrity of the plasma membrane and acrosome morphology. More recently, andrologists are turning to methods that are capable of assessing sperm DNA quality in an attempt to help understand infertility that cannot be explained by traditional sperm parameters. This study has successfully validated and applied a sperm chromatin dispersion test (SCDt) for the assessment of sperm DNA fragmentation of Penaeus monodon. After selecting an appropriate lysing solution, prawn sperm nuclear morphotypes were confirmed with respect to their DNA status using the two-tail comet assay. Once the SCDt methodology was established, we investigated and compared baseline levels of sperm DNA fragmentation (SDF) present in domesticated and wild male broodstock. Additionally, given that mature prawn sperm possess relatively uncondensed chromatin, the susceptibility of the nucleus to mechanical stress (vortex shearing forces) was investigated in an attempt to artificially induce sperm DNA damage. Results revealed that (1) the percentages of SDF indicated by the SCDt were strongly correlated (Pearson R = 0.989; P =.01) with those identified using the two-tailed comet assay, (2) mechanical stress associated with vortexing did not increase SDF level (P =.76) and (3) domesticated male broodstock had a higher (P <.001) SDF level (6.8 ± 4.5%) than that of the wild male broodstock (3.3 ± 1.5%). This study has provided a technique that allows for the assessment of sperm DNA integrity and with further development it could be incorporated as a potential index for male prawn fertility evaluation. We propose that the SCDt can be considered as one of the male fertility parameters in the routine management of male broodstock in prawn aquaculture.
Keyword Comet assay
Decapod crustacean
Shrimp
Sperm DNA fragmentation
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: HERDC Pre-Audit
School of Agriculture and Food Sciences
 
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Created: Fri, 13 Apr 2018, 09:21:31 EST