Computational screening of potential non-immunoglobulin scaffolds using overlapped conserved residues (OCR)-based fingerprints

Munussami, Ganapathiraman, Sokalingam, Sriram, Edwardraja, Selvakumar, Kim, Jung Rae, Chung, Sungwook and Lee, Sun-Gu (2018) Computational screening of potential non-immunoglobulin scaffolds using overlapped conserved residues (OCR)-based fingerprints. Korean Journal of Chemical Engineering, 35 3: 717-724. doi:10.1007/s11814-017-0350-4


Author Munussami, Ganapathiraman
Sokalingam, Sriram
Edwardraja, Selvakumar
Kim, Jung Rae
Chung, Sungwook
Lee, Sun-Gu
Title Computational screening of potential non-immunoglobulin scaffolds using overlapped conserved residues (OCR)-based fingerprints
Journal name Korean Journal of Chemical Engineering   Check publisher's open access policy
ISSN 0256-1115
1975-7220
Publication date 2018-03-16
Sub-type Article (original research)
DOI 10.1007/s11814-017-0350-4
Open Access Status Not yet assessed
Volume 35
Issue 3
Start page 717
End page 724
Total pages 8
Place of publication New York, NY United States
Publisher Springer New York
Language eng
Subject 1600 Chemistry
1500 Chemical Engineering
Abstract Cystatins and lipocalins have attracted considerable interest for their potential applications in non-immunoglobulin protein scaffold engineering. In the present study, their potential homologs were screened computationally from non-redundant protein sequence database based on the overlapped conserved residues (OCR)-fingerprints, which can detect the protein family with low sequence identity, such as cystatins and lipocalins. Two types of OCR-fingerprints for each family were designed and showed very high detection efficiency (>90%). The protein sequence database was scanned by the fingerprints, which yielded the hypothetical sequences for cystatins and lipocalins. The hypothetical sequences were validated further based on their sequence motifs and structural models, which allowed an identification of the potential homologs of cystatins and lipocalins.
Keyword Cystatins
Lipocalins
OCR-fingerprints
Protein Scaffold
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: HERDC Pre-Audit
Institute for Molecular Bioscience - Publications
 
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Created: Fri, 06 Apr 2018, 09:08:08 EST