Tea polyphenols enhance binding of porcine pancreatic α-amylase with starch granules but reduce catalytic activity

Sun, Lijun, Gidley, Michael J. and Warren, Fredrick J. (2018) Tea polyphenols enhance binding of porcine pancreatic α-amylase with starch granules but reduce catalytic activity. Food Chemistry, 258 164-173. doi:10.1016/j.foodchem.2018.03.017


Author Sun, Lijun
Gidley, Michael J.
Warren, Fredrick J.
Title Tea polyphenols enhance binding of porcine pancreatic α-amylase with starch granules but reduce catalytic activity
Journal name Food Chemistry   Check publisher's open access policy
ISSN 1873-7072
0308-8146
Publication date 2018-03-07
Sub-type Article (original research)
DOI 10.1016/j.foodchem.2018.03.017
Open Access Status DOI
Volume 258
Start page 164
End page 173
Total pages 10
Place of publication Amsterdam, NX Netherlands
Publisher Elsevier
Language eng
Subject 1602 Analytical Chemistry
1106 Food Science
Abstract The effects of tea polyphenols on binding of porcine pancreatic α-amylase (PPA) with normal maize starch granules were studied through solution depletion assays, fluorescence spectroscopy and initial rate kinetics. Only polyphenols which have inhibitory activity against PPA increased the binding of PPA with starch. The results are consistent with a binding equilibrium between polyphenols, starch and PPA. The dissociation constant (K) for PPA binding was decreased by tea polyphenols, with the effects greater for theaflavins than catechins and for galloylated than non-galloylated polyphenols. Tea polyphenols were also shown to increase the binding rate of PPA to starch. In addition, there were positive linear correlations between 1/K and reciprocal of competitive inhibition constant (1/K) and between 1/K and fluorescence quenching constant (K). Despite the greater amount of PPA on the granules, starch hydrolysis is reduced because the polyphenol inhibition of PPA persists after binding to starch.
Keyword Binding rate
Dissociation constant
Enzyme binding
Starch granules
Tea polyphenols
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: HERDC Pre-Audit
Queensland Alliance for Agriculture and Food Innovation
 
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Created: Thu, 05 Apr 2018, 22:46:38 EST