The role of CSF1R-dependent macrophages in control of the intestinal stem-cell niche

Sehgal, Anuj, Donaldson, David S., Pridans, Clare, Sauter, Kristin A., Hume, David A. and Mabbott, Neil A. (2018) The role of CSF1R-dependent macrophages in control of the intestinal stem-cell niche. Nature Communications, 9 1: . doi:10.1038/s41467-018-03638-6


Author Sehgal, Anuj
Donaldson, David S.
Pridans, Clare
Sauter, Kristin A.
Hume, David A.
Mabbott, Neil A.
Title The role of CSF1R-dependent macrophages in control of the intestinal stem-cell niche
Journal name Nature Communications   Check publisher's open access policy
ISSN 2041-1723
Publication date 2018-03-28
Year available 2018
Sub-type Article (original research)
DOI 10.1038/s41467-018-03638-6
Open Access Status DOI
Volume 9
Issue 1
Total pages 17
Place of publication London, United Kingdom
Publisher Nature Publishing Group
Language eng
Subject 1600 Chemistry
1300 Biochemistry, Genetics and Molecular Biology
3100 Physics and Astronomy
Abstract Colony-stimulating factor 1 (CSF1) controls the growth and differentiation of macrophages.CSF1R signaling has been implicated in the maintenance of the intestinal stem cell niche and differentiation of Paneth cells, but evidence of expression of CSF1R within the crypt is equivocal. Here we show that CSF1R-dependent macrophages influence intestinal epithelial differentiation and homeostasis. In the intestinal lamina propria CSF1R mRNA expression is restricted to macrophages which are intimately associated with the crypt epithelium, and is undetectable in Paneth cells. Macrophage ablation following CSF1R blockade affects Paneth cell differentiation and leads to a reduction of Lgr5 intestinal stem cells. The disturbances to the crypt caused by macrophage depletion adversely affect the subsequent differentiation of intestinal epithelial cell lineages. Goblet cell density is enhanced, whereas the development of M cells in Peyer's patches is impeded. We suggest that modification of the phenotype or abundance of macrophages in the gut wall alters the development of the intestinal epithelium and the ability to sample gut antigens.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Mater Research Institute-UQ (MRI-UQ)
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Created: Thu, 05 Apr 2018, 22:43:24 EST